Cell Cycle Antibodies 1

Anti-DMTF1 Antibody (CAB17067)

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SKU:
CAB17067
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-DMTF1 Antibody
Antibody SKU:CAB17067
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 561-760 of human DMTF1 (NP_066968.3).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 561-760 of human DMTF1 (NP_066968.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:VTVQ CHTP RVII QTVA TEDI TSSI SQAE LTVD SDIQ SSDF PEPP DALE ADTF PDEI HHPK MTVE PSFN DAHV SKFS DQNS TELM NSVM VRTE EEIS DTDL KQEE SPSD LASA YVTE GLES PTIE EQVD QTID DETI LIVP SPHG FIQA SDVI DTES VLPL TTLT DPIL QHHQ EESN IIGS SLGS PVSE DSKD VEDL VNCH
Gene ID:9988
Uniprot:Q9Y222
Cellular Location:
Calculated MW:
Observed MW:Refer to figures
Synonyms:DMTF1, DMP1, DMTF, MRUL, hDMP1
Background:This gene encodes a transcription factor that contains a cyclin D-binding domain, three central Myb-like repeats, and two flanking acidic transactivation domains at the N- and C-termini. The encoded protein is induced by the oncogenic Ras signaling pathway and functions as a tumor suppressor by activating the transcription of ARF and thus the ARF-p53 pathway to arrest cell growth or induce apoptosis. It also activates the transcription of aminopeptidase N and may play a role in hematopoietic cell differentiation. The transcriptional activity of this protein is regulated by binding of D-cyclins. This gene is hemizygously deleted in approximately 40% of human non-small-cell lung cancer and is a potential prognostic and gene-therapy target for non-small-cell lung cancer. Multiple transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:DMTF1: Transcriptional activator which activates the CDKN2A/ARF locus in response to Ras-Raf signaling, thereby promoting p53/TP53-dependent growth arrest. Binds to the consensus sequence 5'-CCCG[GT]ATGT-3'. Isoform 1 may cooperate with MYB to activate transcription of the ANPEP gene. Isoform 2 may antagonize transcriptional activation by isoform 1. Belongs to the DMTF1 family. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:DNA-binding; Tumor suppressor; Cell cycle regulation

Chromosomal Location of Human Ortholog: 7q21

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function:DNA binding; chromatin binding; transcription factor activity

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; cell cycle

NCBI Summary:This gene encodes a transcription factor that contains a cyclin D-binding domain, three central Myb-like repeats, and two flanking acidic transactivation domains at the N- and C-termini. The encoded protein is induced by the oncogenic Ras signaling pathway and functions as a tumor suppressor by activating the transcription of ARF and thus the ARF-p53 pathway to arrest cell growth or induce apoptosis. It also activates the transcription of aminopeptidase N and may play a role in hematopoietic cell differentiation. The transcriptional activity of this protein is regulated by binding of D-cyclins. This gene is hemizygously deleted in approximately 40% of human non-small-cell lung cancer and is a potential prognostic and gene-therapy target for non-small-cell lung cancer. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]
UniProt Code:Q9Y222
NCBI GenInfo Identifier:74762040
NCBI Gene ID:9988
NCBI Accession:Q9Y222.1
UniProt Secondary Accession:Q9Y222,Q05C48, Q59G79, Q6IS13, Q969T2, Q9H2Z2, Q9H2Z3 B2RBE1, B4DJS5,
UniProt Related Accession:Q9Y222
Molecular Weight:74,878 Da
NCBI Full Name:Cyclin-D-binding Myb-like transcription factor 1
NCBI Synonym Full Names:cyclin D binding myb-like transcription factor 1
NCBI Official Symbol:DMTF1  
NCBI Official Synonym Symbols:DMP1; DMTF; MRUL; hDMP1  
NCBI Protein Information:cyclin-D-binding Myb-like transcription factor 1; hDMTF1; cyclin D-binding Myb-like protein; cyclin-D-interacting Myb-like protein 1
UniProt Protein Name:Cyclin-D-binding Myb-like transcription factor 1
UniProt Synonym Protein Names:Cyclin-D-interacting Myb-like protein 1; hDMP1
Protein Family:Cyclin-D-binding Myb-like transcription factor
UniProt Gene Name:DMTF1  
UniProt Entry Name:DMTF1_HUMAN
Anti-DMTF1 Antibody (CAB17067)Western blot analysis of extracts of HT-29 cells, using DMTF1 antibody (CAB17067) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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