Antibody Name:	Anti-DMP1 Antibody
Antibody SKU:	CAB16832
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human DMP1 (NP_004398.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-937, U-251MG

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human DMP1 (NP_004398.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MKIS ILLM FLWG LSCA LPVT RYQN NESE DSEE WKGH LAQA PTPP LESS ESSE GSKV SSEE QANE DPSD STQS EEGL GSDD HQYI YRLA GGFS RSTG KGGD
Gene ID:	1758
Uniprot:	Q13316
Cellular Location:	Cytoplasm, Nucleus, Secreted, extracellular matrix, extracellular space
Calculated MW:	54kDa/55kDa
Observed MW:	110kDa

Synonyms:

DMP1, ARHP, ARHR, DMP-1

Background:

Dentin matrix acidic phosphoprotein is an extracellular matrix protein and a member of the small integrin binding ligand N-linked glycoprotein family. This protein, which is critical for proper mineralization of bone and dentin, is present in diverse cells of bone and tooth tissues. The protein contains a large number of acidic domains, multiple phosphorylation sites, a functional arg-gly-asp cell attachment sequence, and a DNA binding domain. In undifferentiated osteoblasts it is primarily a nuclear protein that regulates the expression of osteoblast-specific genes. During osteoblast maturation the protein becomes phosphorylated and is exported to the extracellular matrix, where it orchestrates mineralized matrix formation. Mutations in the gene are known to cause autosomal recessive hypophosphatemia, a disease that manifests as rickets and osteomalacia. The gene structure is conserved in mammals. Two transcript variants encoding different isoforms have been described for this gene.

UniProt Protein Function:	DMP1: May have a dual function during osteoblast differentiation. In the nucleus of undifferentiated osteoblasts, unphosphorylated form acts as a transcriptional component for activation of osteoblast-specific genes like osteocalcin. During the osteoblast to osteocyte transition phase it is phosphorylated and exported into the extracellular matrix, where it regulates nucleation of hydroxyapatite. Defects in DMP1 are the cause of rickets hypophosphatemic autosomal recessive type 1 (ARHR1). A hereditary form of hypophosphatemic rickets, a disorder of proximal renal tubule function that causes phosphate loss, hypophosphatemia and skeletal deformities, including rickets and osteomalacia unresponsive to vitamin D. Symptoms are bone pain, fractures and growth abnormalities. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 4q22.1 Cellular Component: extracellular region Molecular Function:calcium ion binding; integrin binding Biological Process: extracellular matrix organization and biogenesis Disease: Hypophosphatemic Rickets, Autosomal Recessive, 1
NCBI Summary:	Dentin matrix acidic phosphoprotein is an extracellular matrix protein and a member of the small integrin binding ligand N-linked glycoprotein family. This protein, which is critical for proper mineralization of bone and dentin, is present in diverse cells of bone and tooth tissues. The protein contains a large number of acidic domains, multiple phosphorylation sites, a functional arg-gly-asp cell attachment sequence, and a DNA binding domain. In undifferentiated osteoblasts it is primarily a nuclear protein that regulates the expression of osteoblast-specific genes. During osteoblast maturation the protein becomes phosphorylated and is exported to the extracellular matrix, where it orchestrates mineralized matrix formation. Mutations in the gene are known to cause autosomal recessive hypophosphatemia, a disease that manifests as rickets and osteomalacia. The gene structure is conserved in mammals. Two transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	Q13316
NCBI GenInfo Identifier:	7673998
NCBI Gene ID:	1758
NCBI Accession:	Q13316.2
UniProt Secondary Accession:	Q13316,O43265, A1L4L3,
UniProt Related Accession:	Q13316
Molecular Weight:	56kDa
NCBI Full Name:	Dentin matrix acidic phosphoprotein 1
NCBI Synonym Full Names:	dentin matrix acidic phosphoprotein 1
NCBI Official Symbol:	DMP1
NCBI Official Synonym Symbols:	ARHP; ARHR; DMP-1
NCBI Protein Information:	dentin matrix acidic phosphoprotein 1
UniProt Protein Name:	Dentin matrix acidic phosphoprotein 1
Protein Family:	Dentin matrix acidic phosphoprotein
UniProt Gene Name:	DMP1

	Western blot analysis of extracts of various cell lines, using DMP1 antibody (CAB16832) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human breast cancer using DMP1 antibody (CAB16832) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse heart using DMP1 antibody (CAB16832) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat testis using DMP1 antibody (CAB16832) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using DMP1 antibody (CAB16832) at dilution of 1:100. Blue: DAPI for nuclear staining.