Cell Biology Antibodies 5

Anti-DLX5 Antibody (CAB15049)

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SKU:
CAB15049
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-DLX5 Antibody
Antibody SKU:CAB15049
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of human DLX5 (NP_005212.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:293T, HeLa, mouse brain, rat brain
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of human DLX5 (NP_005212.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:EMPP EHSP SSSD PMAC NSPQ SPAV WEPQ GSSR SLSH HPHA HPPT SNQS PASS YLEN SASW YTSA ASSI NSHL PPPG SLQH PLAL ASGT LY
Gene ID:1749
Uniprot:P56178
Cellular Location:Nucleus
Calculated MW:20kDa/31kDa
Observed MW:36kDa
Synonyms:DLX5, SHFM1D
Background:This gene encodes a member of a homeobox transcription factor gene family similiar to the Drosophila distal-less gene. The encoded protein may play a role in bone development and fracture healing. Mutation in this gene, which is located in a tail-to-tail configuration with another member of the family on the long arm of chromosome 7, may be associated with split-hand/split-foot malformation.
UniProt Protein Function:DLX5: Transcriptional factor involved in bone development. Acts as an immediate early BMP-responsive transcriptional activator essential for osteoblast differentiation. Stimulates ALPL promoter activity in a RUNX2-independent manner during osteoblast differentiation. Stimulates SP7 promoter activity during osteoblast differentiation. Promotes cell proliferation by up-regulating MYC promoter activity. Involved as a positive regulator of both chondrogenesis and chondrocyte hypertrophy in the endochondral skeleton. Binds to the homeodomain-response element of the ALPL and SP7 promoter. Binds to the MYC promoter. Requires the 5'-TAATTA-3' consensus sequence for DNA-binding. Defects in DLX5 are the cause of split-hand/foot malformation type 1, with sensorineural hearing loss (SHFM1D). A disease characterized by the association of split- hand/foot malformation with deafness. Split-hand/foot malformation is a limb malformation involving the central rays of the autopod and presenting with syndactyly, median clefts of the hands and feet, and aplasia and/or hypoplasia of the phalanges, metacarpals, and metatarsals. Some patients have been found to have mental retardation, ectodermal and craniofacial findings, and orofacial clefting. Belongs to the distal-less homeobox family.
UniProt Protein Details:

Protein type:Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 7q22

Cellular Component: nuclear chromatin

Biological Process: BMP signaling pathway; cell proliferation; endochondral ossification; nervous system development; osteoblast differentiation; positive regulation of transcription, DNA-dependent; skeletal development

Disease: Split-hand/foot Malformation 1 With Sensorineural Hearing Loss, Autosomal Recessive

NCBI Summary:This gene encodes a member of a homeobox transcription factor gene family similiar to the Drosophila distal-less gene. The encoded protein may play a role in bone development and fracture healing. Mutation in this gene, which is located in a tail-to-tail configuration with another member of the family on the long arm of chromosome 7, may be associated with split-hand/split-foot malformation. [provided by RefSeq, Jul 2008]
UniProt Code:P56178
NCBI GenInfo Identifier:12644329
NCBI Gene ID:1749
NCBI Accession:P56178.2
UniProt Secondary Accession:P56178,Q9UPL1, B7Z4P3,
UniProt Related Accession:P56178
Molecular Weight:20,900 Da
NCBI Full Name:Homeobox protein DLX-5
NCBI Synonym Full Names:distal-less homeobox 5
NCBI Official Symbol:DLX5  
NCBI Official Synonym Symbols:SHFM1D  
NCBI Protein Information:homeobox protein DLX-5
UniProt Protein Name:Homeobox protein DLX-5
Protein Family:Homeobox protein
UniProt Gene Name:DLX5  
UniProt Entry Name:DLX5_HUMAN
Anti-DLX5 Antibody (CAB15049)Western blot analysis of extracts of various cell lines, using DLX5 antibody (CAB15049) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90.
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