Metabolism Antibodies 1
Anti-DKK2 Antibody (CAB14874)
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- SKU:
- CAB14874
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-DKK2 Antibody |
| Antibody SKU: | CAB14874 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human DKK2 (NP_055236.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human |
| Positive Samples: | A-549, NCI-H460 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human DKK2 (NP_055236.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | CMVC RRKK KRCH RDGM CCPS TRCN NGIC IPVT ESIL TPHI PALD GTRH RDRN HGHY SNHD LGWQ NLGR PHTK MSHI KGHE GDPC LRSS DCIE GFCC ARHF WTKI CKPV LHQG EVCT KQRK KGSH GLEI FQRC DCAK GLSC KVWK DATY SSKA RLHV CQKI |
| Gene ID: | 27123 |
| Uniprot: | Q9UBU2 |
| Cellular Location: | Secreted |
| Calculated MW: | 28kDa |
| Observed MW: | 30kDa |
| Synonyms: | DKK2, DKK-2 |
| Background: | This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. It can act as either an agonist or antagonist of Wnt/beta-catenin signaling, depending on the cellular context and the presence of the co-factor kremen 2. Activity of this protein is also modulated by binding to the Wnt co-receptor LDL-receptor related protein 6 (LRP6). |
| UniProt Protein Function: | DKK2: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Interacts with LRP5 and LRP6. Expressed in heart, brain, skeletal muscle and lung. Belongs to the dickkopf family. |
| UniProt Protein Details: | Protein type:Secreted; Secreted, signal peptide; Inhibitor Chromosomal Location of Human Ortholog: 4q25 Cellular Component: extracellular space |
| NCBI Summary: | This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. It can act as either an agonist or antagonist of Wnt/beta-catenin signaling, depending on the cellular context and the presence of the co-factor kremen 2. Activity of this protein is also modulated by binding to the Wnt co-receptor LDL-receptor related protein 6 (LRP6). [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9UBU2 |
| NCBI GenInfo Identifier: | 13124093 |
| NCBI Gene ID: | 27123 |
| NCBI Accession: | Q9UBU2.1 |
| UniProt Secondary Accession: | Q9UBU2,Q9UIU3, A0AVE9, B2R6S7, |
| UniProt Related Accession: | Q9UBU2 |
| Molecular Weight: | 28,447 Da |
| NCBI Full Name: | Dickkopf-related protein 2 |
| NCBI Synonym Full Names: | dickkopf WNT signaling pathway inhibitor 2 |
| NCBI Official Symbol: | DKK2 |
| NCBI Official Synonym Symbols: | DKK-2 |
| NCBI Protein Information: | dickkopf-related protein 2 |
| UniProt Protein Name: | Dickkopf-related protein 2 |
| Protein Family: | Dickkopf-related protein |
| UniProt Gene Name: | DKK2 |
| UniProt Entry Name: | DKK2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using DKK2 antibody (CAB14874) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunofluorescence analysis of human skin cancer using DKK2 Rabbit pAb (CAB14874) at dilution of 1:400 (40x lens). Blue: DAPI for nuclear staining. |
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