Epigenetics & Nuclear Signaling Antibodies 2

Anti-DIS3L Antibody (CAB16160)

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SKU:
CAB16160
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-DIS3L Antibody
Antibody SKU:CAB16160
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human DIS3L (NP_588616.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, HeLa, Mouse testis, Rat thymus
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human DIS3L (NP_588616.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MQTA CQAV QHQR GRRQ YNKL RNLL KDAR HDCI LFAN EFQQ CCYL PRER GESM EKWQ TRSI YNAA VWYY HHCQ DRMP IVMV TEDE EAIQ QYGS ETEG VFVI TFKN YLDN FWPD LKAA HELC DSIL QSRR EREN ESQE SHGK EYPE HLPL EVLE AGIK SGRY IQGI LNVN KHRA QIEA FVRL QGAS SKDS DLVS DILI HGMK ARNR SIHG DVVV VELL PKNE WKGR TVAL CEND CDDK ASGE SPSE PMPT GRVV GILQ KNWR DYVV TFPS KEEV QSQG KNAQ KILV TPWD YRIP KIRI STQQ
Gene ID:115752
Uniprot:Q8TF46
Cellular Location:Cytoplasm
Calculated MW:86kDa/105kDa/111kDa/120kDa
Observed MW:130kDa
Synonyms:DIS3L, DIS3L1
Background:The cytoplasmic RNA exosome complex degrades unstable mRNAs and is involved in the regular turnover of other mRNAs. The protein encoded by this gene contains 3'-5' exoribonuclease activity and is a catalytic component of this complex.
UniProt Protein Function:DIS3L: Putative cytoplasm-specific catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. Belongs to the RNR ribonuclease family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 3.1.13.-; Ribonuclease

Chromosomal Location of Human Ortholog: 15q22.31

Cellular Component: centrosome; cytoplasm; plasma membrane

Molecular Function:protein binding; 3'-5'-exoribonuclease activity; enzyme binding; RNA binding

Biological Process: rRNA catabolic process

UniProt Code:Q8TF46
NCBI GenInfo Identifier:219521928
NCBI Gene ID:115752
NCBI Accession:NP_001137160.1
UniProt Secondary Accession:Q8TF46,Q8N1N8, Q8WTU9, Q96CM7,
UniProt Related Accession:Q8TF46
Molecular Weight:120,787 Da
NCBI Full Name:DIS3-like exonuclease 1 isoform 1
NCBI Synonym Full Names:DIS3 like exosome 3'-5' exoribonuclease
NCBI Official Symbol:DIS3L  
NCBI Official Synonym Symbols:DIS3L1  
NCBI Protein Information:DIS3-like exonuclease 1; DIS3 mitotic control homolog-like
UniProt Protein Name:DIS3-like exonuclease 1
Protein Family:DIS3-like exonuclease
UniProt Gene Name:DIS3L  
UniProt Entry Name:DI3L1_HUMAN
Anti-DIS3L Antibody (CAB16160)Western blot analysis of extracts of various cell lines, using DIS3L antibody (CAB16160) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
Anti-DIS3L Antibody (CAB16160)Immunohistochemistry of paraffin-embedded Human liver cancer using DIS3L Rabbit pAb (CAB16160) at dilution of 1:100 (40x lens).
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