Developmental Biology

Anti-DIAPH2 Antibody (CAB10209)

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SKU:
CAB10209
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Developmental Biology

Description

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Antibody Name:Anti-DIAPH2 Antibody
Antibody SKU:CAB10209
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human DIAPH2 (NP_009293.1).
Application:WB
Recommended Dilution:WB 1:1000 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:SKOV3, SW620, NCI-H460, LO2, 293T, Mouse liver, Mouse kidney, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human DIAPH2 (NP_009293.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MEQP GAAA SGAG GGSE EPGG GRSN KRSA GNRA ANEE ETKN KPKL NIQI KTLA DDVR DRIT SFRK STVK KEKP LIQH PIDS QVAM SEFP AAQP LYDE RSLN LSEK EVLD LFEK MMED MNLN
Gene ID:1730
Uniprot:O60879
Cellular Location:Cytoplasm, Early endosome, cytosol
Calculated MW:124kDa/125kDa
Observed MW:125kDa
Synonyms:DIAPH2, DIA, DIA2, DRF2, POF, POF2, POF2A
Background:The product of this gene belongs to the diaphanous subfamily of the formin homology family of proteins. This gene may play a role in the development and normal function of the ovaries. Defects in this gene have been linked to premature ovarian failure 2. Alternatively spliced transcript variants encoding different isoforms have been identified.
UniProt Protein Function:Diaphanous-2: Could be involved in oogenesis. Involved in the regulation of endosome dynamics. Implicated in a novel signal transduction pathway, in which isoform 3 and CSK are sequentially activated by RHOD to regulate the motility of early endosomes through interactions with the actin cytoskeleton. Isoform 3 interacts with RHOD in the GTP-bound form. Expressed in testis, ovary, small intestine, prostate, lung, liver, kidney and leukocytes. Belongs to the formin homology family. Diaphanous subfamily. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: Xq21.33

Cellular Component: cytosol; Golgi apparatus; intracellular membrane-bound organelle; mitochondrion; nucleolus

Molecular Function:receptor binding

Biological Process: cytokinesis; female gamete generation

Disease: Premature Ovarian Failure 2a

NCBI Summary:The product of this gene belongs to the diaphanous subfamily of the formin homology family of proteins. This gene may play a role in the development and normal function of the ovaries. Defects in this gene have been linked to premature ovarian failure 2. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
UniProt Code:O60879
NCBI GenInfo Identifier:6166119
NCBI Gene ID:1730
NCBI Accession:O60879.1
UniProt Secondary Accession:O60879,O60878, Q8WX06, Q8WX48, Q9UJL2, A6NG19,
UniProt Related Accession:O60879
Molecular Weight:125,043 Da
NCBI Full Name:Protein diaphanous homolog 2
NCBI Synonym Full Names:diaphanous related formin 2
NCBI Official Symbol:DIAPH2  
NCBI Official Synonym Symbols:DIA; POF; DIA2; DRF2; POF2  
NCBI Protein Information:protein diaphanous homolog 2
UniProt Protein Name:Protein diaphanous homolog 2
UniProt Synonym Protein Names:Diaphanous-related formin-2; DRF2
Protein Family:Protein diaphanous
UniProt Gene Name:DIAPH2  
UniProt Entry Name:DIAP2_HUMAN
Anti-DIAPH2 Antibody (CAB10209)Western blot analysis of extracts of various cell lines, using DIAPH2 antibody (CAB10209) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
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