Epigenetics & Nuclear Signaling Antibodies 2

Anti-DHX8 Antibody (CAB14724)

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SKU:
CAB14724
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-DHX8 Antibody
Antibody SKU:CAB14724
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1001-1220 of human DHX8 (NP_004932.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:HeLa, LO2, 293T
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1001-1220 of human DHX8 (NP_004932.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:CSEE MLTI VSML SVQN VFYR PKDK QALA DQKK AKFH QTEG DHLT LLAV YNSW KNNK FSNP WCYE NFIQ ARSL RRAQ DIRK QMLG IMDR HKLD VVSC GKST VRVQ KAIC SGFF RNAA KKDP QEGY RTLI DQQV VYIH PSSA LFNR QPEW VVYH ELVL TTKE YMRE VTTI DPRW LVEF APAF FKVS DPTK LSKQ KKQQ RLEP LYNR YEEP NAWR ISRA FRRR
Gene ID:1659
Uniprot:Q14562
Cellular Location:Nucleus
Calculated MW:139kDa
Observed MW:139kDa
Synonyms:DHX8, DDX8, HRH1, PRP22, PRPF22, Dhr2
Background:This gene is a member of the DEAH box polypeptide family. The encoded protein contains the DEAH (Asp-Glu-Ala-His) motif which is characteristic of all DEAH box proteins, and is thought to function as an ATP-dependent RNA helicase that regulates the release of spliced mRNAs from spliceosomes prior to their export from the nucleus. This protein may be required for the replication of human immunodeficiency virus type 1 (HIV-1). Alternative splicing results in multiple transcript variants.
UniProt Protein Function:DDX8: Facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome. Belongs to the DEAD box helicase family. DEAH subfamily. DDX8/PRP22 sub-subfamily.
UniProt Protein Details:

Protein type:Spliceosome; EC 3.6.4.13; RNA processing; RNA splicing; Helicase; RNA-binding

Chromosomal Location of Human Ortholog: 17q21.31

Cellular Component: nucleoplasm; spliceosome; cytoplasm; nucleus

Molecular Function:identical protein binding; protein binding; ATP-dependent RNA helicase activity; ATP binding

Biological Process: RNA processing; nuclear mRNA splicing, via spliceosome; RNA splicing

NCBI Summary:DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is highly homologous to yeast Prp22. This protein facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome. [provided by RefSeq, Jul 2008]
UniProt Code:Q14562
NCBI GenInfo Identifier:4826690
NCBI Gene ID:1659
NCBI Accession:NP_004932.1
UniProt Related Accession:Q14562
Molecular Weight:139,315 Da
NCBI Full Name:ATP-dependent RNA helicase DHX8
NCBI Synonym Full Names:DEAH (Asp-Glu-Ala-His) box polypeptide 8
NCBI Official Symbol:DHX8  
NCBI Official Synonym Symbols:DDX8; HRH1; PRP22; PRPF22  
NCBI Protein Information:ATP-dependent RNA helicase DHX8; RNA helicase HRH1; DEAH box protein 8; DEAH-box protein 8; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 8 (RNA helicase)
UniProt Protein Name:ATP-dependent RNA helicase DHX8
UniProt Synonym Protein Names:DEAH box protein 8; RNA helicase HRH1
Protein Family:ATP-dependent RNA helicase
UniProt Gene Name:DHX8  
UniProt Entry Name:DHX8_HUMAN
Anti-DHX8 Antibody (CAB14724)Western blot analysis of extracts of various cell lines, using DHX8 antibody (CAB14724) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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