Signal Transduction Antibodies 2

Anti-DGKZ Antibody (CAB16457)

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SKU:
CAB16457
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-DGKZ Antibody
Antibody SKU:CAB16457
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 808-1117 of human DGKZ (NP_001099010.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:LO2, HeLa, 293T, Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 808-1117 of human DGKZ (NP_001099010.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:ATMV QKAK RRSA APLH SDQQ PVPE QLRI QVSR VSMH DYEA LHYD KEQL KEAS VPLG TVVV PGDS DLEL CRAH IERL QQEP DGAG AKSP TCQK LSPK WCFL DATT ASRF YRID RAQE HLNY VTEI AQDE IYIL DPEL LGAS ARPD LPTP TSPL PTSP CSPT PRSL QGDA APPQ GEEL IEAA KRND FCKL QELH RAGG DLMH RDEQ SRTL LHHA VSTG SKDV VRYL LDHA PPEI LDAV EENG ETCL HQAA ALGQ RTIC HYIV EAGA SLMK TDQQ GDTP RQRA EKAQ DTEL AAYL ENRQ HYQM IQRE DQET AV
Gene ID:8525
Uniprot:Q13574
Cellular Location:Cell membrane, Cytoplasm, Nucleus
Calculated MW:101kDa/103kDa/104kDa/106kDa/124kDa
Observed MW:120kDa
Synonyms:DGKZ, DAGK5, DAGK6, DGK-ZETA, hDGKzeta
Background:The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It may attenuate protein kinase C activity by regulating diacylglycerol levels in intracellular signaling cascade and signal transduction. Alternative splicing occurs at this locus and multiple transcript variants encoding distinct isoforms have been identified.
UniProt Protein Function:DGKZ: Displays a strong preference for 1,2-diacylglycerols over 1,3-diacylglycerols, but lacks substrate specificity among molecular species of long chain diacylglycerols. Isoform 2 but not isoform 1 regulates RASGRP1 activity. Belongs to the eukaryotic diacylglycerol kinase family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Lipid Metabolism - glycerolipid; Lipid Metabolism - glycerophospholipid; Kinase, lipid; EC 2.7.1.107; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: lamellipodium; nucleoplasm; nucleus; plasma membrane

Molecular Function:diacylglycerol kinase activity; protein binding

Biological Process: platelet activation

NCBI Summary:The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It may attenuate protein kinase C activity by regulating diacylglycerol levels in intracellular signaling cascade and signal transduction. Alternative splicing occurs at this locus and multiple transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Nov 2010]
UniProt Code:Q13574
NCBI GenInfo Identifier:215274170
NCBI Gene ID:8525
NCBI Accession:Q13574.3
UniProt Secondary Accession:Q13574,O00542, Q6ZVG7, Q8IVW9, B7Z2M9, B7Z6M3, E9PPW4 F6UCU9, G3V0F6, J3KNJ6,
UniProt Related Accession:Q13574
Molecular Weight:104,296 Da
NCBI Full Name:Diacylglycerol kinase zeta
NCBI Synonym Full Names:diacylglycerol kinase zeta
NCBI Official Symbol:DGKZ  
NCBI Official Synonym Symbols:DAGK5; DAGK6; DGK-ZETA; hDGKzeta  
NCBI Protein Information:diacylglycerol kinase zeta
UniProt Protein Name:Diacylglycerol kinase zeta
UniProt Synonym Protein Names:Diglyceride kinase zeta; DGK-zeta
Protein Family:Diacylglycerol kinase
UniProt Gene Name:DGKZ  
UniProt Entry Name:DGKZ_HUMAN
Anti-DGKZ Antibody (CAB16457)Western blot analysis of extracts of various cell lines, using DGKZ antibody (CAB16457) at 1:300 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
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