Cell Biology Antibodies 4
Anti-DGKA Antibody (CAB13969)
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- SKU:
- CAB13969
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-DGKA Antibody |
| Antibody SKU: | CAB13969 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human DGKA (NP_958852.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | NCI-H460, MCF7, HeLa, Mouse lung, Rat lung |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human DGKA (NP_958852.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAKE RGLI SPSD FAQL QKYM EYST KKVS DVLK LFED GEMA KYVQ GDAI GYEG FQQF LKIY LEVD NVPR HLSL ALFQ SFET GHCL NETN VTKD VVCL NDVS CYFS LLEG GRPE DKLE FTFK LYDT DRNG ILDS SEVD KIIL QMMR VAEY LDWD VSEL RPIL QEMM KEID YDGS GSVS QAEW VRAG ATTV PLLV LLGL EMTL |
| Gene ID: | 1606 |
| Uniprot: | P23743 |
| Cellular Location: | |
| Calculated MW: | 13kDa/28kDa/82kDa |
| Observed MW: | 83kDa |
| Synonyms: | DGKA, DAGK, DAGK1, DGK-alpha |
| Background: | The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways. It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Several transcript variants encoding different isoforms have been identified for this gene. |
| UniProt Protein Function: | DGKA: Upon cell stimulation converts the second messenger diacylglycerol into phosphatidate, initiating the resynthesis of phosphatidylinositols and attenuating protein kinase C activity. Monomer. Lymphocytes and oligodendroglial cells. Stimulated by calcium and phosphatidylserine. Phosphorylated by protein kinase C. Belongs to the eukaryotic diacylglycerol kinase family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Lipid Metabolism - glycerophospholipid; EC 2.7.1.107; Motility/polarity/chemotaxis; Lipid Metabolism - glycerolipid; Kinase, lipid Chromosomal Location of Human Ortholog: 12q13.3 Cellular Component: membrane; plasma membrane; cytosol Molecular Function:phospholipid binding; calcium ion binding; diacylglycerol kinase activity; ATP binding; NAD+ kinase activity Biological Process: platelet activation; protein kinase C activation; blood coagulation; phosphorylation |
| NCBI Summary: | The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways. It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P23743 |
| NCBI GenInfo Identifier: | 281185505 |
| NCBI Gene ID: | 1606 |
| NCBI Accession: | P23743.3 |
| UniProt Secondary Accession: | P23743,O75481, O75482, O75483, O95217, Q3ZE25, Q8IZ56 Q8N5Q2, |
| UniProt Related Accession: | P23743 |
| Molecular Weight: | Calculated: 13kDa/28kDa/82kDaObserved: 83kDa |
| NCBI Full Name: | Diacylglycerol kinase alpha |
| NCBI Synonym Full Names: | diacylglycerol kinase, alpha 80kDa |
| NCBI Official Symbol: | DGKA |
| NCBI Official Synonym Symbols: | DAGK; DAGK1; DGK-alpha |
| NCBI Protein Information: | diacylglycerol kinase alpha; DAG kinase alpha; diglyceride kinase alpha; 80 kDa diacylglycerol kinase |
| UniProt Protein Name: | Diacylglycerol kinase alpha |
| UniProt Synonym Protein Names: | 80 kDa diacylglycerol kinase; Diglyceride kinase alpha; DGK-alpha |
| Protein Family: | Diacylglycerol kinase |
| UniProt Gene Name: | DGKA |
| UniProt Entry Name: | DGKA_HUMAN |
 | Western blot analysis of extracts of various cell lines, using DGKA antibody (CAB13969) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s. |
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