Cell Biology Antibodies 10

Anti-DGAT1 Antibody (CAB6857)

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SKU:
CAB6857
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-DGAT1 Antibody
Antibody SKU:CAB6857
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 200-300 of human DGAT1 (NP_036211.2).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:HL-60
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 200-300 of human DGAT1 (NP_036211.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:AHTI LFLK LFSY RDVN SWCR RARA KAAS AGKK ASSA AAPH TVSY PDNL TYRD LYYF LFAP TLCY ELNF PRSP RIRK RFLL RRIL EMLF FTQL QVGL IQQW M
Gene ID:8694
Uniprot:O75907
Cellular Location:Endoplasmic reticulum membrane, Multi-pass membrane protein
Calculated MW:55kDa
Observed MW:54kDa
Synonyms:DGAT1, ARAT, ARGP1, DGAT, DIAR7
Background:This gene encodes an multipass transmembrane protein that functions as a key metabolic enzyme. The encoded protein catalyzes the conversion of diacylglycerol and fatty acyl CoA to triacylglycerol. This enzyme can also transfer acyl CoA to retinol. Activity of this protein may be associated with obesity and other metabolic diseases.
UniProt Protein Function:DGAT1: Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. In contrast to DGAT2 it is not essential for survival. May be involved in VLDL (very low density lipoprotein) assembly. In liver, plays a role in esterifying exogenous fatty acids to glycerol. Functions as the major acyl-CoA retinol acyltransferase (ARAT) in the skin, where it acts to maintain retinoid homeostasis and prevent retinoid toxicity leading to skin and hair disorders. Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.
UniProt Protein Details:

Protein type:Transferase; Cofactor and Vitamin Metabolism - retinol; EC 2.3.1.76; Lipid Metabolism - glycerolipid; Membrane protein, multi-pass; EC 2.3.1.20; Membrane protein, integral

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: endoplasmic reticulum membrane; integral to membrane

Molecular Function:2-acylglycerol O-acyltransferase activity; protein binding; transferase activity, transferring acyl groups; retinol O-fatty-acyltransferase activity; diacylglycerol O-acyltransferase activity

Biological Process: sequestering of lipid; triacylglycerol metabolic process; retinol metabolic process; phospholipid metabolic process; fatty acid homeostasis; glycerophospholipid biosynthetic process; triacylglycerol biosynthetic process; diacylglycerol metabolic process; cellular lipid metabolic process

Disease: Diarrhea 7

NCBI Summary:This gene encodes an multipass transmembrane protein that functions as a key metabolic enzyme. The encoded protein catalyzes the conversion of diacylglycerol and fatty acyl CoA to triacylglycerol. This enzyme can also transfer acyl CoA to retinol. Activity of this protein may be associated with obesity and other metabolic diseases. [provided by RefSeq, Jul 2013]
UniProt Code:O75907
NCBI GenInfo Identifier:33302597
NCBI Gene ID:8694
NCBI Accession:O75907.2
UniProt Secondary Accession:O75907,Q96BB8, B2RWQ2, D3DWL6,
UniProt Related Accession:O75907
Molecular Weight:488
NCBI Full Name:Diacylglycerol O-acyltransferase 1
NCBI Synonym Full Names:diacylglycerol O-acyltransferase 1
NCBI Official Symbol:DGAT1  
NCBI Official Synonym Symbols:ARAT; DGAT; ARGP1; DIAR7  
NCBI Protein Information:diacylglycerol O-acyltransferase 1; ACAT related gene product 1; diglyceride acyltransferase; acyl-CoA:diacylglycerol acyltransferase; acyl-CoA retinol O-fatty-acyltransferase; acyl coenzyme A:cholesterol acyltransferase related gene 1
UniProt Protein Name:Diacylglycerol O-acyltransferase 1
UniProt Synonym Protein Names:ACAT-related gene product 1; Acyl-CoA retinol O-fatty-acyltransferase (EC:2.3.1.76); ARAT; Retinol O-fatty-acyltransferase; Diglyceride acyltransferase
Protein Family:Diacylglycerol O-acyltransferase
UniProt Gene Name:DGAT1  
UniProt Entry Name:DGAT1_HUMAN
Anti-DGAT1 Antibody (CAB6857)Western blot analysis of extracts of various cell lines, using DGAT1 antibody (CAB6857) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
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