Antibody Name:	Anti-DEFA1 Antibody
Antibody SKU:	CAB6897
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-94 of human DEFA1 (NP_004075.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse spleen

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-94 of human DEFA1 (NP_004075.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MRTL AILA AILL VALQ AQAE PLQA RADE VAAA PEQI AADI PEVV VSLA WDES LAPK HPGS RKNM ACYC RIPA CIAG ERRY GTCI YQGR LWAF CC
Gene ID:	1667
Uniprot:	P59665
Cellular Location:	Secreted
Calculated MW:	10kDa
Observed MW:	10KDa

Synonyms:

DEFA1, DEF1, DEFA2, HNP-1, HP-1, HP1, MRS

Background:

Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. The protein encoded by this gene, defensin, alpha 1, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. Several alpha defensin genes are clustered on chromosome 8. This gene differs from defensin, alpha 3 by only one amino acid. This gene and the gene encoding defensin, alpha 3 are both subject to copy number variation.

UniProt Protein Function:	defensin, alpha 1: Defensin 1 and defensin 2 have antibacterial, fungicide and antiviral activities. Has antimicrobial activity against Gram- negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. Belongs to the alpha-defensin family.
UniProt Protein Details:	Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 8p23.1 Cellular Component: extracellular matrix; extracellular space; Golgi lumen; extracellular region Biological Process: defense response to Gram-positive bacterium; estrogen receptor signaling pathway; innate immune response in mucosa; killing of cells of another organism; antibacterial humoral response; innate immune response; immune response; chemotaxis; defense response to fungus; defense response to virus
NCBI Summary:	Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. The protein encoded by this gene, defensin, alpha 1, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. Several alpha defensin genes are clustered on chromosome 8. This gene differs from defensin, alpha 3 by only one amino acid. This gene and the gene encoding defensin, alpha 3 are both subject to copy number variation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2014]
UniProt Code:	P59665
NCBI GenInfo Identifier:	30316322
NCBI Gene ID:	728358
NCBI Accession:	P59665.1
UniProt Secondary Accession:	P59665,P11479, Q14125, Q6EZF6,
UniProt Related Accession:	P59665
Molecular Weight:	10,201 Da
NCBI Full Name:	Neutrophil defensin 1
NCBI Synonym Full Names:	defensin, alpha 1B
NCBI Official Symbol:	DEFA1B
NCBI Official Synonym Symbols:	HP1; HP-1; HNP-1
NCBI Protein Information:	neutrophil defensin 1
UniProt Protein Name:	Neutrophil defensin 1
UniProt Synonym Protein Names:	Defensin, alpha 1; HNP-1; HP-1; HP1
UniProt Gene Name:	DEFA1
UniProt Entry Name:	DEF1_HUMAN

	Western blot analysis of extracts of Mouse spleen, using DEFA1 antibody (CAB6897) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s.
	Immunohistochemistry of paraffin-embedded rat heart using DEFA1 antibody (CAB6897) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using DEFA1 antibody (CAB6897). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
DEFA1 Antibody (PACO03567)	Human DEF alpha1 (Defensin Alpha 1, Neutrophil) CLIA Kit (HUES00510)
DEFA1 Antibody (PACO08803)	Human DEF alpha1 (Defensin Alpha 1, Neutrophil) ELISA Kit (HUES01968)
	Human Neutrophil defensin 1 (DEFA1) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)