Antibody Name:	Anti-DDX3X Antibody
Antibody SKU:	CAB5637
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human DDX3X (NP_001347.3).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	C2C12, C6, Mouse brain, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human DDX3X (NP_001347.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSHV AVEN ALGL DQQF AGLD LNSS DNQS GGST ASKG RYIP PHLR NREA TKGF YDKD SSGW SSSK DKDA YSSF GSRS DSRG KSSF FSDR GSGS RGRF DDRG RSDY DGIG SRGD RSGF GKFE RGGN SRWC DKSD EDDW SKPL PPSE RLEQ ELFS GGNT GINF EKYD DIPV EATG NNCP PHIE SFSD VEMG EIIM GNIE LTRY TRPT PVQK HAIP IIKE KRDL
Gene ID:	1654
Uniprot:	O00571
Cellular Location:	Cytoplasm, Mitochondrion outer membrane, Nucleus speckle
Calculated MW:	71kDa/73kDa
Observed MW:	75KDa

Synonyms:

DDX3X, CAP-Rf, DBX, DDX14, DDX3, HLP2, MRX102

Background:

The protein encoded by this gene is a member of the large DEAD-box protein family, that is defined by the presence of the conserved Asp-Glu-Ala-Asp (DEAD) motif, and has ATP-dependent RNA helicase activity. This protein has been reported to display a high level of RNA-independent ATPase activity, and unlike most DEAD-box helicases, the ATPase activity is thought to be stimulated by both RNA and DNA. This protein has multiple conserved domains and is thought to play roles in both the nucleus and cytoplasm. Nuclear roles include transcriptional regulation, mRNP assembly, pre-mRNA splicing, and mRNA export. In the cytoplasm, this protein is thought to be involved in translation, cellular signaling, and viral replication. Misregulation of this gene has been implicated in tumorigenesis. This gene has a paralog located in the nonrecombining region of the Y chromosome. Pseudogenes sharing similarity to both this gene and the DDX3Y paralog are found on chromosome 4 and the X chromosome. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	DDX3: a multifunctional DEAD box family RNA helicase with diverse cellular functions. DEAD box proteins are characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), and are involved in several steps of gene expression, such as transcription, maturation of nuclear and mitochondrial mRNA, mRNA export, translation initiation, and ribosome and spliceosome assembly. DDX3 is required for nuclear export of HIV-1 viral transcripts, possibly in a complex with the viral Rev protein and host cofactor CRM1. DDX3 is required for hepatitis C virus (HCV) RNA replication and its expression is downregulated in hepatitis B virus (HBV) associated hepatocellular carcinoma (HCC). May function as a tumor suppressor protein. Its expression inhibits tumor cell colony formation and increases expression of the cdk inhibitor p21 Waf1/Cip1. Low DDX3 expression has been shown in HCC, and aberrant subcellular localization occurs in many squamous cell carcinomas. Reduced DDX3 expression in cultured cells causes a diminished dependence on serum for cell proliferation and changes in cyclin D1 and p21 Waf1/Cip1 expression. Associates with eIF4F to promote translation of selected mRNAs. Is phosphorylated by the mitotic cyclin dependent kinase, cyclin B/cdc2. This phosphorylation may cause a loss of DDX3 function and a concomitant repression of ribosome biogenesis and translation in mitosis. Involved in TBK1 and IKBKE-dependent IRF3 activation leading to IFN-beta induction. Involved in regulation of apoptosis. May be required for activation of the intrinsic but inhibit activation of the extrinsic apoptotic pathway. Regulated by the cell cycle. Maximally expressed in the cytoplasm during G1/S phase and decreased expression during G2/M phase. Located predominantly in nuclear speckles and, at low levels, throughout the cytoplasm. Associates with the outer side of nuclear pore complexes (NPC) and mitochondrial outer membranes. Shuttles between the nucleus and the cytoplasm in an exportin1-dependent manner. Associates with polyadenylated mRNAs in the cytoplasm and the nucleus. Predominantly located in nucleus during G0 phase and in the cytoplasm during G1/S phase. Belongs to the DEAD box helicase family, DDX3 subfamily. Two isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Spliceosome; Helicase; RNA-binding; Translation initiation; EC 3.6.4.13; Cell cycle regulation Chromosomal Location of Human Ortholog: Xp11.3-p11.23 Cellular Component: eukaryotic translation initiation factor 3 complex; mitochondrial outer membrane; stress granule; cytoplasm; nuclear speck; nucleus Molecular Function:GTPase activity; CTPase activity; RNA binding; ATPase activity; transcription factor binding; poly(A) binding; ATP-dependent DNA helicase activity; protein binding; DNA binding; translation initiation factor binding; nucleoside-triphosphatase activity; eukaryotic initiation factor 4E binding; mRNA 5'-UTR binding; ATP-dependent RNA helicase activity; ribosomal small subunit binding; ATP binding Biological Process: stress granule assembly; positive regulation of viral genome replication; viral reproduction; positive regulation of translation; transcription, DNA-dependent; positive regulation of apoptosis; response to virus; positive regulation of caspase activity; negative regulation of caspase activity; positive regulation of cell growth; DNA duplex unwinding; chromosome segregation; positive regulation of translational initiation; induction of apoptosis via death domain receptors; negative regulation of translation; RNA secondary structure unwinding; positive regulation of interferon-beta production; innate immune response; positive regulation of transcription from RNA polymerase II promoter; negative regulation of cell growth; negative regulation of protein complex assembly; mature ribosome assembly; negative regulation of apoptosis Disease: Mental Retardation, X-linked 102
NCBI Summary:	The protein encoded by this gene is a member of the large DEAD-box protein family, that is defined by the presence of the conserved Asp-Glu-Ala-Asp (DEAD) motif, and has ATP-dependent RNA helicase activity. This protein has been reported to display a high level of RNA-independent ATPase activity, and unlike most DEAD-box helicases, the ATPase activity is thought to be stimulated by both RNA and DNA. This protein has multiple conserved domains and is thought to play roles in both the nucleus and cytoplasm. Nuclear roles include transcriptional regulation, mRNP assembly, pre-mRNA splicing, and mRNA export. In the cytoplasm, this protein is thought to be involved in translation, cellular signaling, and viral replication. Misregulation of this gene has been implicated in tumorigenesis. This gene has a paralog located in the nonrecombining region of the Y chromosome. Pseudogenes sharing similarity to both this gene and the DDX3Y paralog are found on chromosome 4 and the X chromosome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]
UniProt Code:	O00571
NCBI GenInfo Identifier:	3023628
NCBI Gene ID:	1654
NCBI Accession:	O00571.3
UniProt Secondary Accession:	O00571,O15536, A8K538, B4E3E8,
UniProt Related Accession:	O00571
Molecular Weight:	Calculated: 71kDa/73kDaObserved: 80kDa
NCBI Full Name:	ATP-dependent RNA helicase DDX3X
NCBI Synonym Full Names:	DEAD (Asp-Glu-Ala-Asp) box helicase 3, X-linked
NCBI Official Symbol:	DDX3X
NCBI Official Synonym Symbols:	DBX; DDX3; HLP2; DDX14; CAP-Rf
NCBI Protein Information:	ATP-dependent RNA helicase DDX3X; DEAD/H box-3; DEAD box, X isoform; helicase-like protein 2; DEAD box protein 3, X-chromosomal; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3; DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked
UniProt Protein Name:	ATP-dependent RNA helicase DDX3X
UniProt Synonym Protein Names:	DEAD box protein 3, X-chromosomal; DEAD box, X isoform; Helicase-like protein 2; HLP2
Protein Family:	ATP-dependent RNA helicase
UniProt Gene Name:	DDX3X
UniProt Entry Name:	DDX3X_HUMAN

	Western blot analysis of extracts of various cell lines, using DDX3X antibody (CAB5637) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00061). Exposure time: 3s.
	Immunohistochemistry of paraffin-embedded rat brain using DDX3X Rabbit pAb (CAB5637) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse testis using DDX3X Rabbit pAb (CAB5637) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using DDX3X antibody (CAB5637) at dilution of 1:100. Blue: DAPI for nuclear staining.