Antibody Name:	Anti-DDX1 Antibody
Antibody SKU:	CAB6575
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 441-740 of human DDX1 (NP_004930.1).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:10 - 1:100 IP 1:20 - 1:50
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, HepG2, HeLa, 293T, U-251MG, Mouse brain, Mouse skeletal muscle, Rat brain, Rat skeletal muscle

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 441-740 of human DDX1 (NP_004930.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	VHHV VVPV NPKT DRLW ERLG KSHI RTDD VHAK DNTR PGAN SPEM WSEA IKIL KGEY AVRA IKEH KMDQ AIIF CRTK IDCD NLEQ YFIQ QGGG PDKK GHQF SCVC LHGD RKPH ERKQ NLER FKKG DVRF LICT DVAA RGID IHGV PYVI NVTL PDEK QNYV HRIG RVGR AERM GLAI SLVA TEKE KVWY HVCS SRGK GCYN TRLK EDGG CTIW YNEM QLLS EIEE HLNC TISQ VEPD IKVP VDEF DGKV TYGQ KRAA GGGS YKGH VDIL APTV QELA ALEK EAQT SFLH LGYL PNQL FRTF
Gene ID:	1653
Uniprot:	Q92499
Cellular Location:	Cytoplasm, Cytoplasmic granule, Nucleus
Calculated MW:	68kDa/69kDa/82kDa
Observed MW:	100kDa

Synonyms:

DDX1, DBP-RB, UKVH5d

Background:

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein of unknown function. It shows high transcription levels in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin.

UniProt Protein Function:	DDX1: Acts as an ATP-dependent RNA helicase, able to unwind both RNA-RNA and RNA-DNA duplexes. Possesses 5' single-stranded RNA overhang nuclease activity. Possesses ATPase activity on various RNA, but not DNA polynucleotides. May play a role in RNA clearance at DNA double-strand breaks (DSBs), thereby facilitating the template-guided repair of transcriptionally active regions of the genome. Together with RELA, acts as a coactivator to enhance NF-kappa-B-mediated transcriptional activation. Acts as a positive transcriptional regulator of cyclin CCND2 expression. Binds to the cyclin CCND2 promoter region. Associates with chromatin at the NF- kappa-B promoter region via association with RELA. Binds to poly(A) RNA. May be involved in 3'-end cleavage and polyadenylation of pre-mRNAs. Required for HIV-1 Rev function as well as for HIV-1 replication. Binds to the RRE sequence of HIV-1 mRNAs. Belongs to the DEAD box helicase family. DDX1 subfamily.
UniProt Protein Details:	Protein type:EC 3.6.4.13; Helicase; RNA-binding Chromosomal Location of Human Ortholog: 2p24 Cellular Component: nucleoplasm; membrane; stress granule; cytoplasm; ribonucleoprotein complex; nucleus Molecular Function:protein binding; nuclease activity; DNA binding; DNA/RNA helicase activity; exonuclease activity; double-stranded RNA binding; transcription cofactor activity; ATP-dependent helicase activity; chromatin binding; RNA helicase activity; ATP binding; poly(A) binding Biological Process: tRNA splicing; response to exogenous dsRNA; regulation of transcription, DNA-dependent; transcription, DNA-dependent; multicellular organismal development; double-strand break repair; response to virus; spliceosome assembly; DNA duplex unwinding; regulation of translational initiation
NCBI Summary:	DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein of unknown function. It shows high transcription levels in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin. [provided by RefSeq, Jul 2008]
UniProt Code:	Q92499
NCBI GenInfo Identifier:	6919862
NCBI Gene ID:	1653
NCBI Accession:	Q92499.2
UniProt Secondary Accession:	Q92499,B4DME8, B4DPN6,
UniProt Related Accession:	Q92499
Molecular Weight:	740
NCBI Full Name:	ATP-dependent RNA helicase DDX1
NCBI Synonym Full Names:	DEAD (Asp-Glu-Ala-Asp) box helicase 1
NCBI Official Symbol:	DDX1
NCBI Official Synonym Symbols:	DBP-RB; UKVH5d
NCBI Protein Information:	ATP-dependent RNA helicase DDX1; DEAD box-1; DEAD box protein 1; DEAD box polypeptide 1; DEAD box protein retinoblastoma; DEAD (Asp-Glu-Ala-Asp) box polypeptide 1; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 1
UniProt Protein Name:	ATP-dependent RNA helicase DDX1
UniProt Synonym Protein Names:	DEAD box protein 1; DEAD box protein retinoblastoma; DBP-RB
Protein Family:	ATP-dependent RNA helicase
UniProt Gene Name:	DDX1
UniProt Entry Name:	DDX1_HUMAN

	Western blot analysis of extracts of various cell lines, using DDX1 antibody (CAB6575) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded rat brain using DDX1 Antibody (CAB6575) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse lung using DDX1 Antibody (CAB6575) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using DDX1 Antibody (CAB6575) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using DDX1 Antibody (CAB6575) at dilution of 1:100 (40x lens).