KO Validated Antibodies 1

Anti-DDIT4 Antibody (CAB8086)[KO Validated]

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SKU:
CAB8086
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology

Description

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Antibody Name:[KO Validated] DDIT4 Rabbit Polyclonal Antibody
Antibody SKU:CAB8086
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-232 of human DDIT4 (NP_061931.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:A-549, K-562, DU145, MCF-7
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-232 of human DDIT4 (NP_061931.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MPSL WDRF SSSS TSSS PSSL PRTP TPDR PPRS AWGS ATRE EGFD RSTS LESS DCES LDSS NSGF GPEE DTAY LDGV SLPD FELL SDPE DEHL CANL MQLL QESL AQAR LGSR RPAR LLMP SQLV SQVG KELL RLAY SEPC GLRG ALLD VCVE QGKS CHSV GQLA LDPS LVPT FQLT LVLR LDSR LWPK IQGL FSSA NSPF LPGF SQSL TLST GFRV IKKK LYSS EQLL IEEC
Gene ID:54541
Uniprot:Q9NX09
Cellular Location:Cytoplasm, Mitochondrion, cytosol
Calculated MW:25kDa
Observed MW:35kDa
Synonyms:DDIT4, Dig2, REDD-1, REDD1
Background:
UniProt Protein Function:DDIT4: Inhibits cell growth by regulating the TOR signaling pathway upstream of the TSC1-TSC2 complex and downstream of AKT1. Promotes neuronal cell death. Up-regulated in fibroblasts upon ionizing radiation, via a TP53-dependent pathway. Up-regulated by TP63 in primary keratinocytes, and down-regulated during keratinocyte differentiation. Up-regulated upon DNA alkylation. Up-regulated by amyloid beta-peptide and retinoic acid. Up-regulated by hypoxia, via a PI3K and HIF1A-dependent but TP53/TP63-independent mechanism. Broadly expressed, with lowest levels in brain, skeletal muscle and intestine. Up-regulated in substantia nigra neurons from Parkinson disease patients. Belongs to the DDIT4 family.
UniProt Protein Details:

Chromosomal Location of Human Ortholog: 10q22.1

Cellular Component: cytoplasm; cytosol; intracellular

Biological Process: brain development; cell proliferation; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; negative regulation of peptidyl-serine phosphorylation; negative regulation of TOR signaling pathway; nerve growth factor receptor signaling pathway; neuron differentiation; neuron migration; response to hypoxia

UniProt Code:Q9NX09
NCBI GenInfo Identifier:74753036
NCBI Gene ID:54541
NCBI Accession:Q9NX09.1
UniProt Secondary Accession:Q9NX09,Q9H0S3,
UniProt Related Accession:Q9NX09
Molecular Weight:25,371 Da
NCBI Full Name:DNA damage-inducible transcript 4 protein
NCBI Synonym Full Names:DNA damage inducible transcript 4
NCBI Official Symbol:DDIT4  
NCBI Official Synonym Symbols:Dig2; REDD1; REDD-1  
NCBI Protein Information:DNA damage-inducible transcript 4 protein
UniProt Protein Name:DNA damage-inducible transcript 4 protein
UniProt Synonym Protein Names:HIF-1 responsive protein RTP801; Protein regulated in development and DNA damage response 1; REDD-1
Protein Family:DNA damage-inducible transcript 4 protein
UniProt Gene Name:DDIT4  
UniProt Entry Name:DDIT4_HUMAN
Anti-DDIT4 Polyclonal Antibody [KO Validated] (CAB8086)Western blot analysis of extracts of various cell lines, using DDIT4 antibody (CAB8086) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-DDIT4 Polyclonal Antibody [KO Validated] (CAB8086)Western blot analysis of extracts from normal (control) and DDIT4 knockout (KO) HeLa cells, using DDIT4 antibody (CAB8086) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
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