Epigenetics & Nuclear Signaling Antibodies 5
Anti-DDB2 Antibody (CAB11615)
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- SKU:
- CAB11615
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Monoclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-DDB2 Antibody |
| Antibody SKU: | CAB11615 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | A synthesized peptide derived from human DDB2 |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | HeLa, U-87MG, Mouse lung |
| Immunogen: | A synthesized peptide derived from human DDB2 |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 1643 |
| Uniprot: | Q92466 |
| Cellular Location: | |
| Calculated MW: | 45kDa |
| Observed MW: | 48KDa |
 | Western blot - DDB2 Rabbit mAb (CAB11615) |
| Synonyms: | DDBB, UV-DDB2, XPE |
| Background: | This gene encodes a protein that is necessary for the repair of ultraviolet light-damaged DNA. This protein is the smaller subunit of a heterodimeric protein complex that participates in nucleotide excision repair, and this complex mediates the ubiquitylation of histones H3 and H4, which facilitates the cellular response to DNA damage. This subunit appears to be required for DNA binding. Mutations in this gene cause xeroderma pigmentosum complementation group E, a recessive disease that is characterized by an increased sensitivity to UV light and a high predisposition for skin cancer development, in some cases accompanied by neurological abnormalities. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014] |
| UniProt Protein Function: | DDB2: Required for DNA repair. Binds to DDB1 to form the UV- damaged DNA-binding protein complex (the UV-DDB complex). The UV- DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as the substrate recognition module for the DCX (DDB1- CUL4-X-box) E3 ubiquitin-protein ligase complex DDB1-CUL4-ROC1 (also known as CUL4-DDB-ROC1 and CUL4-DDB-RBX1). The DDB1-CUL4- ROC1 complex may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. The DDB1-CUL4-ROC1 complex also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. Isoform D1 and isoform D2 inhibit UV-damaged DNA repair. Component of the UV-DDB complex which includes DDB1 and DDB2. The UV-DDB complex interacts with monoubiquitinated histone H2A and binds to XPC via the DDB2 subunit. Component of the DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex DDB1-CUL4- ROC1 (also known as CUL4-DDB-ROC1 and CUL4-DDB-RBX1), which includes CUL4A or CUL4B, DDB1, DDB2 and RBX1. DDB2 may function as the substrate recognition module within this complex. The DDB1- CUL4-ROC1 complex may associate with the COP9 signalosome, and this inhibits the E3 ubiquitin-protein ligase activity of the complex. A large number of other DCX complexes may also exist in which an alternate substrate targeting subunit replaces DDB2. These targeting subunits are generally known as DCAF (DDB1- and CUL4-associated factor) or CDW (CUL4-DDB1-associated WD40-repeat) proteins. Isoform D1 and isoform D2 do not interact with DDB1. Expression is induced in response to treatment with IR or UV and this requires p53/TP53 activity. Ubiquitously expressed; with highest levels in corneal endothelium and lowest levels in brain. Isoform D1 is highly expressed in brain and heart. Isoform D2, isoform D3 and isoform D4 are weakly expressed. Belongs to the WD repeat DDB2/WDR76 family. 5 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:DNA repair, damage Chromosomal Location of Human Ortholog: 11p12-p11 Cellular Component: nucleoplasm; protein complex; cell junction Molecular Function:protein binding; DNA binding; damaged DNA binding; ubiquitin-protein ligase activity Biological Process: protein autoubiquitination; protein polyubiquitination; nucleotide-excision repair; pyrimidine dimer repair; nucleotide-excision repair, DNA damage removal; DNA repair; response to UV Disease: Xeroderma Pigmentosum, Complementation Group E |
| NCBI Summary: | This gene encodes a protein that is necessary for the repair of ultraviolet light-damaged DNA. This protein is the smaller subunit of a heterodimeric protein complex that participates in nucleotide excision repair, and this complex mediates the ubiquitylation of histones H3 and H4, which facilitates the cellular response to DNA damage. This subunit appears to be required for DNA binding. Mutations in this gene cause xeroderma pigmentosum complementation group E, a recessive disease that is characterized by an increased sensitivity to UV light and a high predisposition for skin cancer development, in some cases accompanied by neurological abnormalities. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014] |
| UniProt Code: | Q92466 |
| NCBI GenInfo Identifier: | 12230033 |
| NCBI Gene ID: | 1643 |
| NCBI Accession: | Q92466.1 |
| UniProt Secondary Accession: | Q92466,Q76E54, Q76E55, Q76E56, Q76E57, B2R875, |
| UniProt Related Accession: | Q92466 |
| Molecular Weight: | 427 |
| NCBI Full Name: | DNA damage-binding protein 2 |
| NCBI Synonym Full Names: | damage-specific DNA binding protein 2, 48kDa |
| NCBI Official Symbol: | DDB2 |
| NCBI Official Synonym Symbols: | DDBB; UV-DDB2 |
| NCBI Protein Information: | DNA damage-binding protein 2; UV-DDB 2; DDB p48 subunit; UV-damaged DNA-binding protein 2; damage-specific DNA-binding protein 2; xeroderma pigmentosum group E protein |
| UniProt Protein Name: | DNA damage-binding protein 2 |
| UniProt Synonym Protein Names: | DDB p48 subunit; DDBb; Damage-specific DNA-binding protein 2; UV-damaged DNA-binding protein 2; UV-DDB 2 |
| Protein Family: | DNA damage-binding protein |
| UniProt Gene Name: | DDB2 |
| UniProt Entry Name: | DDB2_HUMAN |
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