Cell Biology Antibodies 10
Anti-DDAH2 Antibody (CAB6457)
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- SKU:
- CAB6457
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-DDAH2 Antibody |
| Antibody SKU: | CAB6457 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-285 of human DDAH2 (NP_039268.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:10 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SW620, BT-474, MCF7, Mouse lung, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-285 of human DDAH2 (NP_039268.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MGTP GEGL GRCS HALI RGVP ESLA SGEG AGAG LPAL DLAK AQRE HGVL GGKL RQRL GLQL LELP PEES LPLG PLLG DTAV IQGD TALI TRPW SPAR RPEV DGVR KALQ DLGL RIVE IGDE NATL DGTD VLFT GREF FVGL SKWT NHRG AEIV ADTF RDFA VSTV PVSG PSHL RGLC GMGG PRTV VAGS SDAA QKAV RAMA VLTD HPYA SLTL PDDA AADC LFLR PGLP GVPP FLLH RGGG DLPN SQEA LQKL SDVT LVPV SCSE LEKA GAGL SSLC LVLS TRPH S |
| Gene ID: | 23564 |
| Uniprot: | O95865 |
| Cellular Location: | Cytoplasm, Mitochondrion |
| Calculated MW: | 29kDa |
| Observed MW: | 30kDa |
| Synonyms: | DDAH2, DDAH, DDAHII, G6a, HEL-S-277, NG30 |
| Background: | This gene encodes a dimethylarginine dimethylaminohydrolase. The encoded enzyme functions in nitric oxide generation by regulating the cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. The protein may be localized to the mitochondria. Alternative splicing resulting in multiple transcript variants. |
| UniProt Protein Function: | DDAH2: Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation. Belongs to the DDAH family. |
| UniProt Protein Details: | Protein type:EC 3.5.3.18; Apoptosis; Hydrolase Chromosomal Location of Human Ortholog: 6p21.3 Cellular Component: centrosome; mitochondrion; cytoplasm Molecular Function:amino acid binding; protein binding; dimethylargininase activity; catalytic activity Biological Process: positive regulation of nitric oxide biosynthetic process; citrulline metabolic process; arginine catabolic process; nitric oxide biosynthetic process; nitric oxide mediated signal transduction; negative regulation of apoptosis |
| NCBI Summary: | This gene encodes a dimethylarginine dimethylaminohydrolase. The encoded enzyme functions in nitric oxide generation by regulating the cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. The protein may be localized to the mitochondria. Alternative splicing resulting in multiple transcript variants. [provided by RefSeq, Dec 2014] |
| UniProt Code: | O95865 |
| NCBI GenInfo Identifier: | 6831529 |
| NCBI Gene ID: | 23564 |
| NCBI Accession: | O95865.1 |
| UniProt Related Accession: | O95865 |
| Molecular Weight: | ~ 30kDa |
| NCBI Full Name: | N(G),N(G)-dimethylarginine dimethylaminohydrolase 2 |
| NCBI Synonym Full Names: | dimethylarginine dimethylaminohydrolase 2 |
| NCBI Official Symbol: | DDAH2 |
| NCBI Official Synonym Symbols: | G6a; DDAH; NG30; DDAHII; HEL-S-277 |
| NCBI Protein Information: | N(G),N(G)-dimethylarginine dimethylaminohydrolase 2 |
| UniProt Protein Name: | N(G),N(G)-dimethylarginine dimethylaminohydrolase 2 |
| UniProt Synonym Protein Names: | DDAHII; Dimethylargininase-2; Protein G6a; S-phase protein |
| Protein Family: | N(G),N(G)-dimethylarginine dimethylaminohydrolase |
| UniProt Gene Name: | DDAH2 |
| UniProt Entry Name: | DDAH2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using DDAH2 antibody (CAB6457) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunofluorescence analysis of U2OS cells using DDAH2 antibody (CAB6457). Blue: DAPI for nuclear staining. |
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