Cell Biology Antibodies 5

Anti-DCN Antibody (CAB15048)

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SKU:
CAB15048
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-DCN Antibody
Antibody SKU:CAB15048
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 31-359 of human DCN (NP_598010.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, Mouse heart, Rat heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 31-359 of human DCN (NP_598010.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:DEAS GIGP EVPD DRDF EPSL GPVC PFRC QCHL RVVQ CSDL GLDK VPKD LPPD TTLL DLQN NKIT EIKD GDFK NLKN LHAL ILVN NKIS KVSP GAFT PLVK LERL YLSK NQLK ELPE KMPK TLQE LRAH ENEI TKVR KVTF NGLN QMIV IELG TNPL KSSG IENG AFQG MKKL SYIR IADT NITS IPQG LPPS LTEL HLDG NKIS RVDA ASLK GLNN LAKL GLSF NSIS AVDN GSLA NTPH LREL HLDN NKLT RVPG GLAE HKYI QVVY LHNN NISV VGSS DFCP PGHN TKKA SYSG VSLF SNPV QYWE IQPS TFRC VYVR SAIQ LGNY K
Gene ID:1634
Uniprot:P07585
Cellular Location:Secreted, extracellular matrix, extracellular space
Calculated MW:8kDa/19kDa/23kDa/27kDa/39kDa
Observed MW:45KDa/48KDa
Synonyms:DCN, CSCD, DSPG2, PG40, PGII, PGS2, SLRR1B, decorin, Decorin
Background:This gene encodes a member of the small leucine-rich proteoglycan family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protein. This protein plays a role in collagen fibril assembly. Binding of this protein to multiple cell surface receptors mediates its role in tumor suppression, including a stimulatory effect on autophagy and inflammation and an inhibitory effect on angiogenesis and tumorigenesis. This gene and the related gene biglycan are thought to be the result of a gene duplication. Mutations in this gene are associated with congenital stromal corneal dystrophy in human patients.
UniProt Protein Function:DCN: May affect the rate of fibrils formation. Defects in DCN are the cause of congenital stromal corneal dystrophy (CSCD). Corneal dystrophies are inherited, bilateral, primary alterations of the cornea that are not associated with prior inflammation or secondary to systemic disease. Most show autosomal dominant inheritance. Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Secreted, signal peptide; Extracellular matrix; Secreted; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 12q21.33

Cellular Component: extracellular matrix; lysosomal lumen; proteinaceous extracellular matrix; extracellular space; Golgi lumen; extracellular region; collagen type VI

Molecular Function:collagen binding; glycosaminoglycan binding; extracellular matrix binding; protein N-terminus binding

Biological Process: extracellular matrix organization and biogenesis; skeletal muscle development; chondroitin sulfate biosynthetic process; glycosaminoglycan metabolic process; wound healing; response to lipopolysaccharide; pathogenesis; dermatan sulfate biosynthetic process; chondroitin sulfate metabolic process; extracellular matrix disassembly; organ morphogenesis; response to mechanical stimulus; chondroitin sulfate catabolic process; carbohydrate metabolic process; kidney development; peptide cross-linking via chondroitin 4-sulfate glycosaminoglycan; aging; placenta development

Disease: Corneal Dystrophy, Congenital Stromal

NCBI Summary:The protein encoded by this gene is a small cellular or pericellular matrix proteoglycan that is closely related in structure to biglycan protein. The encoded protein and biglycan are thought to be the result of a gene duplication. This protein is a component of connective tissue, binds to type I collagen fibrils, and plays a role in matrix assembly. It contains one attached glycosaminoglycan chain. This protein is capable of suppressing the growth of various tumor cell lines. There are multiple alternatively spliced transcript variants known for this gene. This gene is a candidate gene for Marfan syndrome. [provided by RefSeq, Jul 2008]
UniProt Code:P07585
NCBI GenInfo Identifier:129951
NCBI Gene ID:1634
NCBI Accession:P07585.1
UniProt Secondary Accession:P07585,Q9P0Z0, Q9P0Z1, Q9Y5N8, Q9Y5N9,
UniProt Related Accession:P07585
Molecular Weight:8,266 Da
NCBI Full Name:Decorin
NCBI Synonym Full Names:decorin
NCBI Official Symbol:DCN  
NCBI Official Synonym Symbols:CSCD; PG40; PGII; PGS2; DSPG2; SLRR1B  
NCBI Protein Information:decorin; PG-S2; bone proteoglycan II; decorin proteoglycan; proteoglycan core protein; small leucine-rich protein 1B; dermatan sulphate proteoglycans II
UniProt Protein Name:Decorin
UniProt Synonym Protein Names:Bone proteoglycan II; PG-S2; PG40
Protein Family:Decorin
UniProt Gene Name:DCN  
UniProt Entry Name:PGS2_HUMAN
Anti-DCN Antibody (CAB15048)Western blot analysis of extracts of various cell lines, using DCN antibody (CAB15048) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
Anti-DCN Antibody (CAB15048)Western blot analysis of extracts of HeLa cells, using DCN antibody (CAB15048) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-DCN Antibody (CAB15048)Immunofluorescence analysis of NIH-3T3 cells using DCN Rabbit pAb (CAB15048) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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