Name: Anti-DCLRE1B Antibody (CAB6808)
Brand: Epigenetics & Nuclear Signaling Antibodies 4
SKU: CAB6808
Availability: OutOfStock

Antibody Name:	Anti-DCLRE1B Antibody
Antibody SKU:	CAB6808
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 323-532 of human DCLRE1B (NP_073747.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	THP-1

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 323-532 of human DCLRE1B (NP_073747.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	VQQY MSSS SRKP SLLW LLER RLKR PRTQ GVVF ESPE ESAD QSQA DRDS KKAK KEKL SPWP ADLE KQPS HHPL RIKK QLFP DLYS KEWN KAVP FCES QKRV TMLT APLG FSVH LRST DEEF ISQK TREE IGLG SPLV PMGD DDGG PEAT GNQS AWMG HGSP LSHS SKGT PLLA TEFR GLAL KYLL TPVN FFQA GYSS RRFD QQVE KYHK PC
Gene ID:	64858
Uniprot:	Q9H816
Cellular Location:	Chromosome, Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center, telomere
Calculated MW:	60kDa
Observed MW:	60kDa

Synonyms:	DCLRE1B, APOLLO, SNM1B, SNMIB
Background:

UniProt Protein Function:	DCLRE1B: 5'-3' exonuclease that plays a central role in telomere maintenance and protection during S-phase. Participates in the protection of telomeres against non-homologous end-joining (NHEJ)- mediated repair, thereby ensuring that telomeres do not fuse. Plays a key role in telomeric loop (T loop) formation by being recruited by TERF2 at the leading end telomeres and by processing leading-end telomeres immediately after their replication via its exonuclease activity: generates 3' single-stranded overhang at the leading end telomeres avoiding blunt leading-end telomeres that are vulnerable to end-joining reactions and expose the telomere end in a manner that activates the DNA repair pathways. Together with TERF2, required to protect telomeres from replicative damage during replication by controlling the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology. Also involved in response to DNA damage: plays a role in response to DNA interstrand cross-links (ICLs) by facilitating double-strand break formation. In case of spindle stress, involved in prophase checkpoint. Defects in DCLRE1B may be a cause of Hoyeraal-Hreidarsson syndrome (HHS). HHS is a multisystem disorder affecting males and is characterized by aplastic anemia, immunodeficiency, microcephaly, cerebellar hypoplasia, and growth retardation. An aberrant splice variant designated Apollo- Delta has been found in a patient with Hoyeraal-Hreidarsson syndrome. Apollo-Delta hampers the proper replication of telomeres, leading to major telomeric dysfunction and cellular senescence, but maintains its DNA interstrand cross-link repair function in the whole genome. Belongs to the DNA repair metallo-beta-lactamase (DRMBL) family.
UniProt Protein Details:	Protein type:Hydrolase; DNA repair, damage; EC 3.1.-.- Chromosomal Location of Human Ortholog: 1p13.2 Cellular Component: centrosome; chromosome, telomeric region; cytoplasm; nucleus Molecular Function:5'-3' exonuclease activity; protein binding Biological Process: telomeric loop formation; telomeric 3' overhang formation; DNA repair; telomere maintenance; cell cycle checkpoint; protection from non-homologous end joining at telomere
NCBI Summary:	DNA interstrand cross-links prevent strand separation, thereby physically blocking transcription, replication, and segregation of DNA. DCLRE1B is one of several evolutionarily conserved genes involved in repair of interstrand cross-links (Dronkert et al., 2000 [PubMed 10848582]).[supplied by OMIM, Mar 2008]
UniProt Code:	Q9H816
NCBI GenInfo Identifier:	73620756
NCBI Gene ID:	64858
NCBI Accession:	Q9H816.1
UniProt Secondary Accession:	Q9H816,Q9H9E5,
UniProt Related Accession:	Q9H816
Molecular Weight:	532
NCBI Full Name:	5' exonuclease Apollo
NCBI Synonym Full Names:	DNA cross-link repair 1B
NCBI Official Symbol:	DCLRE1B
NCBI Official Synonym Symbols:	SNM1B; SNMIB; APOLLO
NCBI Protein Information:	5' exonuclease Apollo; PSO2 homolog; SNM1 homolog B; DNA cross-link repair 1B (PSO2 homolog, S. cerevisiae)
UniProt Protein Name:	5' exonuclease Apollo
UniProt Synonym Protein Names:	DNA cross-link repair 1B protein; SNM1 homolog B; SNMIB; hSNM1B
Protein Family:	5' exonuclease
UniProt Gene Name:	DCLRE1B
UniProt Entry Name:	DCR1B_HUMAN

Western blot analysis of extracts of THP-1 cells, using DCLRE1B antibody (CAB6808) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

Antibodies

DCLRE1B Antibody (PACO03286)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)