Metabolism Antibodies 2

Anti-DBH Antibody (CAB2711)

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SKU:
CAB2711
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism

Description

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Antibody Name:Anti-DBH Antibody
Antibody SKU:CAB2711
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 150-250 of human DBH (NP_000778.3).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:1000 IHC 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse brain
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 150-250 of human DBH (NP_000778.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:PFGT CDPK DYLI EDGT VHLV YGIL EEPF RSLE AING SGLQ MGLQ RVQL LKPN IPEP ELPS DACT MEVQ APNI QIPS QETT YWCY IKEL PKGF SRHH IIKY E
Gene ID:1621
Uniprot:P09172
Cellular Location:Cytoplasmic vesicle, Single-pass type II membrane protein, chromaffin granule lumen, chromaffin granule membrane, secretory vesicle, secretory vesicle lumen, secretory vesicle membrane
Calculated MW:69kDa
Observed MW:79kDa
Synonyms:DBH, DBM
Background:The protein encoded by this gene is an oxidoreductase belonging to the copper type II, ascorbate-dependent monooxygenase family. It is present in the synaptic vesicles of postganglionic sympathetic neurons and converts dopamine to norepinephrine. It exists in both soluble and membrane-bound forms, depending on the absence or presence, respectively, of a signal peptide.
UniProt Protein Function:DBH: Conversion of dopamine to noradrenaline. Homotetramer composed of two non-covalently bound disulfide-linked dimers. Activity is enhanced by nerve growth factor (in superior cervical ganglia and adrenal medulla). Trans-synaptic stimulation with reserpine, acetylcholine and glucocorticoids. Belongs to the copper type II ascorbate-dependent monooxygenase family.
UniProt Protein Details:

Protein type:Membrane protein, integral; Motility/polarity/chemotaxis; EC 1.14.17.1; Oxidoreductase; Apoptosis; Amino Acid Metabolism - tyrosine; Cell cycle regulation

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: membrane; cytoplasm; integral to membrane; extracellular region

Molecular Function:copper ion binding; L-ascorbic acid binding; dopamine beta-monooxygenase activity; catalytic activity

Biological Process: fear response; dopamine catabolic process; maternal behavior; response to amphetamine; cytokine production; locomotory behavior; response to pain; behavioral response to ethanol; leukocyte mediated immunity; glucose homeostasis; memory; regulation of cell proliferation; norepinephrine biosynthetic process; synaptic transmission; catecholamine biosynthetic process; homoiothermy; positive regulation of vasoconstriction; visual learning; blood vessel remodeling; leukocyte migration

Disease: Dopamine Beta-hydroxylase Deficiency, Congenital

NCBI Summary:The protein encoded by this gene is an oxidoreductase belonging to the copper type II, ascorbate-dependent monooxygenase family. The encoded protein, expressed in neuroscretory vesicles and chromaffin granules of the adrenal medulla, catalyzes the conversion of dopamine to norepinephrine, which functions as both a hormone and as the main neurotransmitter of the sympathetic nervous system. The enzyme encoded by this gene exists exists in both soluble and membrane-bound forms, depending on the absence or presence, respectively, of a signal peptide. Mutations in this gene cause dopamine beta-hydroxylate deficiency in human patients, characterized by deficits in autonomic and cardiovascular function, including hypotension and ptosis. Polymorphisms in this gene may play a role in a variety of psychiatric disorders. [provided by RefSeq, Aug 2017]
UniProt Code:P09172
NCBI GenInfo Identifier:158517849
NCBI Gene ID:1621
NCBI Accession:P09172.3
UniProt Related Accession:P09172
Molecular Weight:
NCBI Full Name:Dopamine beta-hydroxylase
NCBI Synonym Full Names:dopamine beta-hydroxylase
NCBI Official Symbol:DBH  
NCBI Official Synonym Symbols:DBM; ORTHYP1  
NCBI Protein Information:dopamine beta-hydroxylase
UniProt Protein Name:Dopamine beta-hydroxylase
UniProt Synonym Protein Names:Dopamine beta-monooxygenaseSoluble dopamine beta-hydroxylase
Protein Family:Dopamine beta-hydroxylase
UniProt Gene Name:DBH  
UniProt Entry Name:DOPO_HUMAN
Anti-DBH Antibody (CAB2711)Western blot analysis of extracts of mouse brain, using DBH antibody (CAB2711) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 30s.
Anti-DBH Antibody (CAB2711)Immunohistochemistry of paraffin-embedded human stomach using DBH antibody (CAB2711) at dilution of 1:100 (40x lens).
Anti-DBH Antibody (CAB2711)Immunohistochemistry of paraffin-embedded rat heart using DBH antibody (CAB2711) at dilution of 1:100 (40x lens).
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