Antibody Name:	Anti-DAZL Antibody
Antibody SKU:	CAB1114
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 186-295 of human DAZL (NP_001342.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	MCF7, SH-SY5Y, HL-60, SGC-7901, Mouse brain, Mouse stomach

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 186-295 of human DAZL (NP_001342.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	VYNY QMPP QWPV GEQR SYVV PPAY SAVN YHCN EVDP GAEV VPNE CSVH EATP PSGN GPQK KSVD RSIQ TVVS CLFN PENR LRNS VVTQ DDYF KDKR VHHF RRSR AMLK SV
Gene ID:	1618
Uniprot:	Q92904
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	33kDa/35kDa
Observed MW:	33kDa

Synonyms:

DAZL, DAZH, DAZL1, DAZLA, SPGYLA

Background:

The DAZ (Deleted in AZoospermia) gene family encodes potential RNA binding proteins that are expressed in prenatal and postnatal germ cells of males and females. The protein encoded by this gene is localized to the nucleus and cytoplasm of fetal germ cells and to the cytoplasm of developing oocytes. In the testis, this protein is localized to the nucleus of spermatogonia but relocates to the cytoplasm during meiosis where it persists in spermatids and spermatozoa. Transposition and amplification of this autosomal gene during primate evolution gave rise to the DAZ gene cluster on the Y chromosome. Mutations in this gene have been linked to severe spermatogenic failure and infertility in males. Two transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	DAZL: RNA-binding protein, which is essential for gametogenesis. Plays a central role during spermatogenesis. May act by binding to the 3'-UTR of mRNA and thereby regulating the translation of key transcripts. Homodimer and heterodimer. Forms a heterodimer with DAZ. Interacts with BOLL, DAZAP1 and DAZAP2. Interacts with PUM2. Testis specific. Belongs to the RRM DAZ family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Translation Chromosomal Location of Human Ortholog: 3p24.3 Cellular Component: polysome; cytoplasm; nucleus Molecular Function:identical protein binding; protein binding; translation activator activity; mRNA 3'-UTR binding; RNA binding; nucleotide binding Biological Process: positive regulation of translational initiation; positive regulation of meiosis; female meiosis II; multicellular organismal development; spermatogenesis; oocyte maturation; germ cell development
NCBI Summary:	The DAZ (Deleted in AZoospermia) gene family encodes potential RNA binding proteins that are expressed in prenatal and postnatal germ cells of males and females. The protein encoded by this gene is localized to the nucleus and cytoplasm of fetal germ cells and to the cytoplasm of developing oocytes. In the testis, this protein is localized to the nucleus of spermatogonia but relocates to the cytoplasm during meiosis where it persists in spermatids and spermatozoa. Transposition and amplification of this autosomal gene during primate evolution gave rise to the DAZ gene cluster on the Y chromosome. Mutations in this gene have been linked to severe spermatogenic failure and infertility in males. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]
UniProt Code:	Q92904
NCBI GenInfo Identifier:	27805453
NCBI Gene ID:	1618
NCBI Accession:	Q92904.1
UniProt Secondary Accession:	Q92904,O15396, Q5HYB4, Q92909,
UniProt Related Accession:	Q92904
Molecular Weight:	295
NCBI Full Name:	Deleted in azoospermia-like
NCBI Synonym Full Names:	deleted in azoospermia-like
NCBI Official Symbol:	DAZL
NCBI Official Synonym Symbols:	DAZH; DAZL1; DAZLA; SPGYLA
NCBI Protein Information:	deleted in azoospermia-like; DAZ homolog; DAZ-like autosomal; SPGY-like-autosomal; deleted in azoospermia-like 1; germline specific RNA binding protein; spermatogenesis gene on the Y-like autosomal
UniProt Protein Name:	Deleted in azoospermia-like
UniProt Synonym Protein Names:	DAZ homolog; DAZ-like autosomal; Deleted in azoospermia-like 1; SPGY-like-autosomal
Protein Family:	Deleted in azoospermia
UniProt Gene Name:	DAZL
UniProt Entry Name:	DAZL_HUMAN

	Western blot analysis of extracts of various cell lines, using DAZL antibody (CAB1114) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of HeLa cells using DAZL antibody (CAB1114) at dilution of 1:100. Blue: DAPI for nuclear staining.