Metabolism Antibodies 2
Anti-DARS2 Antibody (CAB7813)
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- SKU:
- CAB7813
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-DARS2 Antibody |
| Antibody SKU: | CAB7813 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 406-645 of human DARS2 (NP_060592.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | NCI-H460, U-251MG, MCF7, BT-474, Mouse liver, Mouse heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 406-645 of human DARS2 (NP_060592.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | ANRN WNSP VANF IMES QRLE LIRL METQ EEDV VLLT AGEH NKAC SLLG KLRL ECAD LLET RGVV LRDP TLFS FLWV VDFP LFLP KEEN PREL ESAH HPFT APHP SDIH LLYT EPKK ARSQ HYDL VLNG NEIG GGSI RIHN AELQ RYIL ATLL KEDV KMLS HLLQ ALDY GAPP HGGI ALGL DRLI CLVT GSPS IRDV IAFP KSFR GHDL MSNT PDSV PPEE LKPY HIRV SKPT DSKA ERAH |
| Gene ID: | 55157 |
| Uniprot: | Q6PI48 |
| Cellular Location: | Mitochondrion matrix |
| Calculated MW: | 73kDa |
| Observed MW: | 74kDa |
| Synonyms: | DARS2, ASPRS, LBSL, MT-ASPRS |
| Background: | The protein encoded by this gene belongs to the class-II aminoacyl-tRNA synthetase family. It is a mitochondrial enzyme that specifically aminoacylates aspartyl-tRNA. Mutations in this gene are associated with leukoencephalopathy with brainstem and spinal cord involvement and lactate elevation (LBSL). |
| UniProt Protein Function: | DARS2: Defects in DARS2 are a cause of leukoencephalopathy with brainstem and spinal cord involvement and lactate elevation (LBSL). LBSL is an autosomal recessive disease and is defined on the basis of a highly characteristic constellation of abnormalities observed by magnetic resonance imaging and spectroscopy. Affected individuals develop slowly progressive cerebellar ataxia, spasticity, and dorsal column dysfunction, sometimes with a mild cognitive deficit or decline. Belongs to the class-II aminoacyl-tRNA synthetase family. |
| UniProt Protein Details: | Protein type:Ligase; Translation; EC 6.1.1.12; Mitochondrial Chromosomal Location of Human Ortholog: 1q25.1 Cellular Component: nucleoplasm; mitochondrion; mitochondrial matrix; nucleus Molecular Function:protein binding; protein homodimerization activity; aspartate-tRNA ligase activity; aspartate-tRNA(Asn) ligase activity; ATP binding; tRNA binding Biological Process: tRNA aminoacylation for protein translation; gene expression; tRNA aminoacylation Disease: Leukoencephalopathy With Brainstem And Spinal Cord Involvement And Lactate Elevation |
| NCBI Summary: | The protein encoded by this gene belongs to the class-II aminoacyl-tRNA synthetase family. It is a mitochondrial enzyme that specifically aminoacylates aspartyl-tRNA. Mutations in this gene are associated with leukoencephalopathy with brainstem and spinal cord involvement and lactate elevation (LBSL). [provided by RefSeq, Nov 2009] |
| UniProt Code: | Q6PI48 |
| NCBI GenInfo Identifier: | 74758347 |
| NCBI Gene ID: | 55157 |
| NCBI Accession: | Q6PI48.1 |
| UniProt Related Accession: | Q6PI48 |
| Molecular Weight: | Observed: 68 kDaPredicted: 74 kDa |
| NCBI Full Name: | Aspartate--tRNA ligase, mitochondrial |
| NCBI Synonym Full Names: | aspartyl-tRNA synthetase 2, mitochondrial |
| NCBI Official Symbol: | DARS2 |
| NCBI Official Synonym Symbols: | LBSL; ASPRS; mtAspRS; MT-ASPRS |
| NCBI Protein Information: | aspartate--tRNA ligase, mitochondrial |
| UniProt Protein Name: | Aspartate--tRNA ligase, mitochondrial |
| UniProt Synonym Protein Names: | Aspartyl-tRNA synthetase; AspRS |
| UniProt Gene Name: | DARS2 |
| UniProt Entry Name: | SYDM_HUMAN |
 | Western blot analysis of extracts of various cell lines, using DARS2 antibody (CAB7813) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human liver damage using DARS2 antibody (CAB7813) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human lung cancer using DARS2 antibody (CAB7813) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human stomach using DARS2 antibody (CAB7813) at dilution of 1:100 (40x lens). |
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