Description
Antibody Name: | Anti-D27 Antibody (CAB20706) |
Antibody SKU: | CAB20706 |
Antibody Size: | 50µL, 100µL |
Application: | Western blotting |
Reactivity: | Oryza sativa |
Host Species: | Rabbit |
Immunogen: | Recombinant protein of Oryza sativa D27. |
Application: | Western blotting |
Recommended Dilution: | WB 1:500 - 1:2000 |
Reactivity: | Oryza sativa |
Positive Samples: | stems, spikes |
Immunogen: | Recombinant protein of Oryza sativa D27. |
Purification Method: | Affinity purification |
Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Isotype: | IgG |
Sequence: | Email for sequence |
Cellular Location: | |
Calculated MW: | 31kDa |
Observed MW: | 31kDa |
Synonyms: | D27 |
Background: | Involved in strigolactones biosynthesis by catalyzing the isomerization of the C9-C10 double bond in all-trans-beta-carotene leading to 9-cis-beta-carotene and providing the substrate for CCD7. Strigolactones are hormones that inhibit tillering and shoot branching through the MAX-dependent pathway, contribute to the regulation of shoot architectural response to phosphate-limiting conditions and function as rhizosphere signals that stimulate hyphal branching of arbuscular mycorrhizal fungi and trigger seed germination of root parasitic weeds. |
![]() | Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L. ) variety Zhonghua 11, using D27 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 120s. |