Signal Transduction Antibodies 2

Anti-CYTH4 Antibody (CAB14875)

(No reviews yet) Write a Review
SKU:
CAB14875
Product Type:
Antibody
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-CYTH4 Antibody
Antibody SKU:CAB14875
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-70 of human CYTH4 (NP_037517.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse, Rat
Positive Samples:Mouse liver, Mouse brain, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-70 of human CYTH4 (NP_037517.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MDLC HPEP AELS SGET EELQ RIKW HRKQ LLED IQKL KDEI ADVF AQID CFES AEES RMAQ KEKE LCIG RK
Gene ID:27128
Uniprot:Q9UIA0
Cellular Location:Cell membrane, Peripheral membrane protein
Calculated MW:45kDa
Observed MW:46kDa
Synonyms:CYTH4, CYT4, DJ63G5.1, PSCD4
Background:This gene encodes a member of the PSCD family of proteins, which have an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein (GEP) activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family function as GEPs for ADP-ribosylation factors (ARFs), which are guanine nucleotide-binding proteins involved in vesicular trafficking pathways. This protein exhibits GEP activity in vitro with ARF1 and ARF5, but is inactive with ARF6. Alternatively spliced transcript variants have been found for this gene.
UniProt Protein Function:cytohesin 4: Promotes guanine-nucleotide exchange on ARF1 and ARF5. Promotes the activation of ARF through replacement of GDP with GTP.
UniProt Protein Details:

Protein type:GEFs; GEFs, ARF

Chromosomal Location of Human Ortholog: 22q12.3-q13.1

Cellular Component: cytosol; Golgi membrane; plasma membrane

Molecular Function:ARF guanyl-nucleotide exchange factor activity

NCBI Summary:This gene encodes a member of the PSCD family of proteins, which have an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein (GEP) activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family function as GEPs for ADP-ribosylation factors (ARFs), which are guanine nucleotide-binding proteins involved in vesicular trafficking pathways. This protein exhibits GEP activity in vitro with ARF1 and ARF5, but is inactive with ARF6. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2015]
UniProt Code:Q9UIA0
NCBI GenInfo Identifier:13124094
NCBI Gene ID:27128
NCBI Accession:Q9UIA0.1
UniProt Secondary Accession:Q9UIA0,Q5R3F9, Q9UGT6,
UniProt Related Accession:Q9UIA0
Molecular Weight:45,672 Da
NCBI Full Name:Cytohesin-4
NCBI Synonym Full Names:cytohesin 4
NCBI Official Symbol:CYTH4  
NCBI Official Synonym Symbols:CYT4; PSCD4; DJ63G5.1  
NCBI Protein Information:cytohesin-4
UniProt Protein Name:Cytohesin-4
UniProt Synonym Protein Names:PH, SEC7 and coiled-coil domain-containing protein 4
Protein Family:Cytohesin
UniProt Gene Name:CYTH4  
UniProt Entry Name:CYH4_HUMAN
Anti-CYTH4 Antibody (CAB14875)Western blot analysis of extracts of various cell lines, using CYTH4 antibody (CAB14875) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
View AllClose

0 Reviews

View AllClose

Related Products