Antibody Name:	CST6 Rabbit Polyclonal Antibody
Antibody SKU:	CAB8115
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 29-149 of human CST6 (NP_001314.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human
Positive Samples:	293T, MCF7

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 29-149 of human CST6 (NP_001314.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	RPQE RMVG ELRD LSPD DPQV QKAA QAAV ASYN MGSN SIYY FRDT HIIK AQSQ LVAG IKYF LTME MGST DCRK TRVT GDHV DLTT CPLA AGAQ QEKL RCDF EVLV VPWQ NSSQ LLKH NCVQ M
Gene ID:	1474
Uniprot:	Q15828
Cellular Location:	Secreted
Calculated MW:	16kDa
Observed MW:	15kDa

Synonyms:

CST6

Background:

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. This gene encodes a cystatin from the type 2 family, which is down-regulated in metastatic breast tumor cells as compared to primary tumor cells. Loss of expression is likely associated with the progression of a primary tumor to a metastatic phenotype.

UniProt Protein Function:	CST6: Shows moderate inhibition of cathepsin B but is not active against cathepsin C. Belongs to the cystatin family.
UniProt Protein Details:	Protein type:Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide Chromosomal Location of Human Ortholog: 11q13 Cellular Component: cornified envelope Molecular Function:cysteine protease inhibitor activity Biological Process: epidermis development; anatomical structure morphogenesis
NCBI Summary:	The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. This gene encodes a cystatin from the type 2 family, which is down-regulated in metastatic breast tumor cells as compared to primary tumor cells. Loss of expression is likely associated with the progression of a primary tumor to a metastatic phenotype. [provided by RefSeq, Jul 2008]
UniProt Code:	Q15828
NCBI GenInfo Identifier:	4503113
NCBI Gene ID:	1474
NCBI Accession:	NP_001314.1
UniProt Secondary Accession:	Q15828,Q540N7,
UniProt Related Accession:	Q15828
Molecular Weight:	16,511 Da
NCBI Full Name:	cystatin-M
NCBI Synonym Full Names:	cystatin E/M
NCBI Official Symbol:	CST6
NCBI Protein Information:	cystatin-M; cystatin 6; cystatin M; cystatin-6; cystatin-E; cystatin M/E; cysteine proteinase inhibitor
UniProt Protein Name:	Cystatin-M
UniProt Synonym Protein Names:	Cystatin-6; Cystatin-E
Protein Family:	Cystatin
UniProt Gene Name:	CST6
UniProt Entry Name:	CYTM_HUMAN

	Western blot analysis of extracts of various cell lines, using CST6 antibody (CAB8115) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.
	Immunofluorescence analysis of U2OS cells using CST6 antibody (CAB8115) at dilution of 1:100. Blue: DAPI for nuclear staining.