Cell Cycle Antibodies 1

Anti-Cyclin E1 Antibody (CAB14225)

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SKU:
CAB14225
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-Cyclin E1 Antibody
Antibody SKU:CAB14225
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 300-410 of human Cyclin E1 (NP_001229.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:MCF7, K-562, Mouse brain, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 300-410 of human Cyclin E1 (NP_001229.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:AASA LYHF SSSE LMQK VSGY QWCD IENC VKWM VPFA MVIR ETGS SKLK HFRG VADE DAHN IQTH RDSL DLLD KARA KKAM LSEQ NRAS PLPS GLLT PPQS GKKQ SSGP EMA
Gene ID:898
Uniprot:P24864
Cellular Location:Nucleus
Calculated MW:47kDa
Observed MW:47KDa
Synonyms:CCNE1, CCNE, pCCNE1, cyclin E1
Background:The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB.
UniProt Protein Function:CCNE1: a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. Accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Two alternatively spliced isoforms have been described.
UniProt Protein Details:

Protein type:Nuclear receptor co-regulator; Cell cycle regulation; Activator

Chromosomal Location of Human Ortholog: 19q12

Cellular Component: cytosol; nucleoplasm; nucleus

Molecular Function:protein binding

Biological Process: G1/S transition of mitotic cell cycle; G1/S-specific transcription in mitotic cell cycle; protein amino acid phosphorylation

NCBI Summary:The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. [provided by RefSeq, Apr 2016]
UniProt Code:P24864
NCBI GenInfo Identifier:3041657
NCBI Gene ID:898
NCBI Accession:P24864.2
UniProt Secondary Accession:P24864,Q14091, Q8NFG1, Q92501, Q9UD21, A8K684,
UniProt Related Accession:P24864
Molecular Weight:45,150 Da
NCBI Full Name:G1/S-specific cyclin-E1
NCBI Synonym Full Names:cyclin E1
NCBI Official Symbol:CCNE1  
NCBI Official Synonym Symbols:CCNE; pCCNE1  
NCBI Protein Information:G1/S-specific cyclin-E1
UniProt Protein Name:G1/S-specific cyclin-E1
UniProt Gene Name:CCNE1  
UniProt Entry Name:CCNE1_HUMAN
Anti-Cyclin E1 Antibody (CAB14225)Western blot analysis of extracts of various cell lines, using Cyclin E1 antibody (CAB14225) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-Cyclin E1 Antibody (CAB14225)Immunohistochemistry of paraffin-embedded mouse lung using Cyclin E1 Rabbit pAb (CAB14225) at dilution of 1:150 (40x lens).
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