Cell Biology Antibodies 9
Anti-CYB5A Antibody (CAB5401)
(No reviews yet)
Write a Review
- SKU:
- CAB5401
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-CYB5A Antibody |
| Antibody SKU: | CAB5401 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human CYB5A (NP_683725.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | MCF7, A-549, HT-29, 293T, M21 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human CYB5A (NP_683725.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAEQ SDEA VKYY TLEE IQKH NHSK STWL ILHH KVYD LTKF LEEH PGGE EVLR EQAG GDAT ENFE DVGH STDA REMS KTFI IGEL HPDD RPKL NKPP ETLI |
| Gene ID: | 1528 |
| Uniprot: | P00167 |
| Cellular Location: | Cytoplasm, Cytoplasmic side, Endoplasmic reticulum membrane, Microsome membrane, Single-pass membrane protein |
| Calculated MW: | 11kDa/14kDa/15kDa |
| Observed MW: | 17kDa |
| Synonyms: | CYB5A, CYB5, MCB5 |
| Background: | The protein encoded by this gene is a membrane-bound cytochrome that reduces ferric hemoglobin (methemoglobin) to ferrous hemoglobin, which is required for stearyl-CoA-desaturase activity. Defects in this gene are a cause of type IV hereditary methemoglobinemia. Three transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | CYB5A: Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases. Defects in CYB5A are the cause of methemoglobinemia CYB5A-related (METHB-CYB5A). A form of methemoglobinemia, a hematologic disease characterized by the presence of excessive amounts of methemoglobin in blood cells, resulting in decreased oxygen carrying capacity of the blood, cyanosis and hypoxia. Belongs to the cytochrome b5 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 18q23 Cellular Component: endoplasmic reticulum membrane; mitochondrial outer membrane; membrane; integral to membrane Molecular Function:cytochrome-c oxidase activity; enzyme binding; metal ion binding; aldo-keto reductase activity; heme binding Biological Process: vitamin metabolic process; response to cadmium ion; L-ascorbic acid metabolic process; water-soluble vitamin metabolic process Disease: Methemoglobinemia Type Iv |
| NCBI Summary: | The protein encoded by this gene is a membrane-bound cytochrome that reduces ferric hemoglobin (methemoglobin) to ferrous hemoglobin, which is required for stearyl-CoA-desaturase activity. Defects in this gene are a cause of type IV hereditary methemoglobinemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010] |
| UniProt Code: | P00167 |
| NCBI GenInfo Identifier: | 117809 |
| NCBI Gene ID: | 1528 |
| NCBI Accession: | P00167.2 |
| UniProt Secondary Accession: | P00167,Q6IB14, A8MV91, F8WEU4, |
| UniProt Related Accession: | P00167 |
| Molecular Weight: | 14,169 Da |
| NCBI Full Name: | Cytochrome b5 |
| NCBI Synonym Full Names: | cytochrome b5 type A (microsomal) |
| NCBI Official Symbol: | CYB5A |
| NCBI Official Synonym Symbols: | CYB5; MCB5 |
| NCBI Protein Information: | cytochrome b5; type 1 cyt-b5 |
| UniProt Protein Name: | Cytochrome b5 |
| UniProt Synonym Protein Names: | Microsomal cytochrome b5 type A; MCB5 |
| Protein Family: | Cytochrome |
| UniProt Gene Name: | CYB5A |
| UniProt Entry Name: | CYB5_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CYB5A antibody (CAB5401) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 120s. |
 | Immunohistochemistry of paraffin-embedded rat kidney using CYB5A antibody (CAB5401) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse heart using CYB5A antibody (CAB5401) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of C6 cells using CYB5A Polyclonal Antibody (CAB5401) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
View AllClose
Related Products
