Immunology Antibodies 2
Anti-CUL1 Antibody (CAB2136)
- SKU:
- CAB2136
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-CUL1 Antibody |
| Antibody SKU: | CAB2136 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 500-776 of human CUL1 (NP_003583.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:10 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | K-562 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 500-776 of human CUL1 (NP_003583.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | YTSK LQRM FQDI GVSK DLNE QFKK HLTN SEPL DLDF SIQV LSSG SWPF QQSC TFAL PSEL ERSY QRFT AFYA SRHS GRKL TWLY QLSK GELV TNCF KNRY TLQA STFQ MAIL LQYN TEDA YTVQ QLTD STQI KMDI LAQV LQIL LKSK LLVL EDEN ANVD EVEL KPDT LIKL YLGY KNKK LRVN INVP MKTE QKQE QETT HKNI EEDR KLLI QAAI VRIM KMRK VLKH QQLL GEVL TQLS SRFK PRVP VIKK CIDI LIEK EYLE RVDG EKDT YSYL A |
| Gene ID: | 8454 |
| Uniprot: | Q13616 |
| Cellular Location: | |
| Calculated MW: | 89kDa |
| Observed MW: | 100kDa |
| Synonyms: | Cullin 1, CUL1, cullin-1, MGC149834, MGC149835 |
| Background: |
| UniProt Protein Function: | CUL1: a core scaffolding component of multiple cullin-RING-based SCF (SKP1- CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. The cullin subunit serves as a rigid scaffold of SCF complexes. May contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex depends on the neddylation of the cullin subunit. Interacts with multiple F-box proteins in SCF complexes, directing the ubiquitination of many substrates. These F-box proteins and associated substrates include: BTRC and FBXW11 bind CTNNB1 and participate in Wnt signaling; FBXW11 bind phosphorylated NFKBIA; BTRC binds NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5 and probably NFKB2; SKP2 binds phosphorylated p27kip and is involved in regulation of G1/S transition; SKP2 binds ORC1, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1; FBXW7 binds cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1; FBXW2 binds GCM1; FBXO32 binds MYOD1; FBXO7 binds BIRC2 and DLGAP5; FBXO33 binds YBX1; and Cyclin F binds CP110. Interacts with CHEK2, mediating its ubiquitination and regulating its function. Belongs to the cullin family. |
| UniProt Protein Details: | Protein type:Ubiquitin conjugating system; Cell cycle regulation Chromosomal Location of Human Ortholog: 7q36.1 Cellular Component: nucleoplasm; cullin-RING ubiquitin ligase complex; SCF ubiquitin ligase complex; cytosol Molecular Function:protein binding; ubiquitin protein ligase binding Biological Process: protein monoubiquitination; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; circadian rhythm; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; Notch signaling pathway; viral reproduction; protein ubiquitination; negative regulation of cell proliferation; cell proliferation; organ morphogenesis; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle; cell cycle arrest; G2/M transition of mitotic cell cycle; G1/S transition of mitotic cell cycle |
| UniProt Code: | Q13616 |
| NCBI GenInfo Identifier: | 19863257 |
| NCBI Gene ID: | 8454 |
| NCBI Accession: | Q13616.2 |
| UniProt Secondary Accession: | Q13616,O60719, Q08AL6, Q8IYW1, D3DWG3, |
| UniProt Related Accession: | Q13616 |
| Molecular Weight: | 89,679 Da |
| NCBI Full Name: | Cullin-1 |
| NCBI Synonym Full Names: | cullin 1 |
| NCBI Official Symbol: | CUL1 |
| NCBI Protein Information: | cullin-1; CUL-1 |
| UniProt Protein Name: | Cullin-1 |
| Protein Family: | Cullin |
| UniProt Gene Name: | CUL1 |
| UniProt Entry Name: | CUL1_HUMAN |
 | Western blot analysis of extracts of K-562 cells, using CUL1 antibody (CAB2136) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunofluorescence analysis of HeLa cells using CUL1 antibody (CAB2136). Blue: DAPI for nuclear staining. |
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