Cell Biology Antibodies 2

Anti-CTSL Antibody (CAB12066)

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SKU:
CAB12066
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-CTSL Antibody
Antibody SKU:CAB12066
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 114-333 of human CTSL (NP_001903.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:LO2, 293T
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 114-333 of human CTSL (NP_001903.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:APRS VDWR EKGY VTPV KNQG QCGS CWAF SATG ALEG QMFR KTGR LISL SEQN LVDC SGPQ GNEG CNGG LMDY AFQY VQDN GGLD SEES YPYE ATEE SCKY NPKY SVAN DTGF VDIP KQEK ALMK AVAT VGPI SVAI DAGH ESFL FYKE GIYF EPDC SSED MDHG VLVV GYGF ESTE SDNN KYWL VKNS WGEE WGMG GYVK MAKD RRNH CGIA SAAS YPTV
Gene ID:1514
Uniprot:P07711
Cellular Location:Lysosome
Calculated MW:37kDa
Observed MW:38kDa
Synonyms:CTSL, CATL, CTSL1, MEP
Background:The protein encoded by this gene is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. Multiple alternatively spliced transcript variants have been found for this gene.
UniProt Protein Function:Important for the overall degradation of proteins in lysosomes.
NCBI Summary:The protein encoded by this gene is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Apr 2012]
UniProt Code:P07711
NCBI GenInfo Identifier:115741
NCBI Gene ID:1514
NCBI Accession:P07711.2
UniProt Secondary Accession:P07711,Q6IAV1, Q96QJ0,
UniProt Related Accession:P07711
Molecular Weight:38kDa
NCBI Full Name:Cathepsin L1
NCBI Synonym Full Names:cathepsin L
NCBI Official Symbol:CTSL  
NCBI Official Synonym Symbols:MEP; CATL; CTSL1  
NCBI Protein Information:cathepsin L1
UniProt Protein Name:Cathepsin L1
UniProt Synonym Protein Names:Cathepsin L; Major excreted protein; MEP
UniProt Gene Name:CTSL  
Anti-CTSL Antibody (CAB12066)Western blot analysis of extracts of various cell lines, using CTSL antibody (CAB12066) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-CTSL Antibody (CAB12066)Immunofluorescence analysis of C6 cells using CTSL antibody (CAB12066) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-CTSL Antibody (CAB12066)Immunofluorescence analysis of NIH/3T3 cells using CTSL antibody (CAB12066) at dilution of 1:100. Blue: DAPI for nuclear staining.
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