Antibody Name:	Anti-CTSF Antibody
Antibody SKU:	CAB10469
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 270-484 of human CTSF (NP_003784.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	U-251MG, SKOV3

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 270-484 of human CTSF (NP_003784.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LAPP EWDW RSKG AVTK VKDQ GMCG SCWA FSVT GNVE GQWF LNQG TLLS LSEQ ELLD CDKM DKAC MGGL PSNA YSAI KNLG GLET EDDY SYQG HMQS CNFS AEKA KVYI NDSV ELSQ NEQK LAAW LAKR GPIS VAIN AFGM QFYR HGIS RPLR PLCS PWLI DHAV LLVG YGNR SDVP FWAI KNSW GTDW GEKG YYYL HRGS GACG VNTM ASSA VVD
Gene ID:	8722
Uniprot:	Q9UBX1
Cellular Location:	Lysosome
Calculated MW:	53kDa
Observed MW:	53kDa

Synonyms:

CTSF, CATSF, CLN13

Background:

Cathepsins are papain family cysteine proteinases that represent a major component of the lysosomal proteolytic system. Cathepsins generally contain a signal sequence, followed by a propeptide and then a catalytically active mature region. The very long (251 amino acid residues) proregion of the cathepsin F precursor contains a C-terminal domain similar to the pro-segment of cathepsin L-like enzymes, a 50-residue flexible linker peptide, and an N-terminal domain predicted to adopt a cystatin-like fold. The cathepsin F proregion is unique within the papain family cysteine proteases in that it contains this additional N-terminal segment predicted to share structural similarities with cysteine protease inhibitors of the cystatin superfamily. This cystatin-like domain contains some of the elements known to be important for inhibitory activity. CTSF encodes a predicted protein of 484 amino acids which contains a 19 residue signal peptide. Cathepsin F contains five potential N-glycosylation sites, and it may be targeted to the endosomal/lysosomal compartment via the mannose 6-phosphate receptor pathway. The cathepsin F gene is ubiquitously expressed, and it maps to chromosome 11q13, close to the gene encoding cathepsin W.

UniProt Protein Function:	CTSF: Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis. Belongs to the peptidase C1 family.
UniProt Protein Details:	Protein type:EC 3.4.22.41; Protease Chromosomal Location of Human Ortholog: 11q13 Cellular Component: extracellular space; lysosomal lumen; lysosome Molecular Function:cysteine-type endopeptidase activity Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; proteolysis; proteolysis involved in cellular protein catabolic process Disease: Ceroid Lipofuscinosis, Neuronal, 13
NCBI Summary:	Cathepsins are papain family cysteine proteinases that represent a major component of the lysosomal proteolytic system. Cathepsins generally contain a signal sequence, followed by a propeptide and then a catalytically active mature region. The very long (251 amino acid residues) proregion of the cathepsin F precursor contains a C-terminal domain similar to the pro-segment of cathepsin L-like enzymes, a 50-residue flexible linker peptide, and an N-terminal domain predicted to adopt a cystatin-like fold. The cathepsin F proregion is unique within the papain family cysteine proteases in that it contains this additional N-terminal segment predicted to share structural similarities with cysteine protease inhibitors of the cystatin superfamily. This cystatin-like domain contains some of the elements known to be important for inhibitory activity. CTSF encodes a predicted protein of 484 amino acids which contains a 19 residue signal peptide. Cathepsin F contains five potential N-glycosylation sites, and it may be targeted to the endosomal/lysosomal compartment via the mannose 6-phosphate receptor pathway. The cathepsin F gene is ubiquitously expressed, and it maps to chromosome 11q13, close to the gene encoding cathepsin W. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9UBX1
NCBI GenInfo Identifier:	12643325
NCBI Gene ID:	8722
NCBI Accession:	Q9UBX1.1
UniProt Secondary Accession:	Q9UBX1,O95240, Q9NSU4, Q9UKQ5, B2R964,
UniProt Related Accession:	Q9UBX1
Molecular Weight:	53,366 Da
NCBI Full Name:	Cathepsin F
NCBI Synonym Full Names:	cathepsin F
NCBI Official Symbol:	CTSF
NCBI Official Synonym Symbols:	CATSF; CLN13
NCBI Protein Information:	cathepsin F
UniProt Protein Name:	Cathepsin F
Protein Family:	Cathepsin
UniProt Gene Name:	CTSF
UniProt Entry Name:	CATF_HUMAN

	Western blot analysis of extracts of various cell lines, using CTSF antibody (CAB10469) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded human breast cancer using CTSF antibody (CAB10469) at dilution of 1:100 (40x lens).