Cell Biology Antibodies 3

Anti-CTSD Antibody (CAB13292)

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SKU:
CAB13292
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-CTSD Antibody
Antibody SKU:CAB13292
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 65-412 of human CTSD (NP_001900.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HepG2, MCF7, rat liver, rat brain, mouse spleen, mouse brain, mouse kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 65-412 of human CTSD (NP_001900.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:GPIP EVLK NYMD AQYY GEIG IGTP PQCF TVVF DTGS SNLW VPSI HCKL LDIA CWIH HKYN SDKS STYV KNGT SFDI HYGS GSLS GYLS QDTV SVPC QSAS SASA LGGV KVER QVFG EATK QPGI TFIA AKFD GILG MAYP RISV NNVL PVFD NLMQ QKLV DQNI FSFY LSRD PDAQ PGGE LMLG GTDS KYYK GSLS YLNV TRKA YWQV HLDQ VEVA SGLT LCKE GCEA IVDT GTSL MVGP VDEV RELQ KAIG AVPL IQGE YMIP CEKV STLP AITL KLGG KGYK LSPE DYTL KVSQ AGKT LCLS GFMG MDIP PPSG PLWI LGDV FIGR YYTV FDRD NNRV GFAE AARL
Gene ID:1509
Uniprot:P07339
Cellular Location:Lysosome, Melanosome, Secreted, extracellular space
Calculated MW:44kDa
Observed MW:15kDa/28kDa/43kDa/46kDa
Synonyms:CLN10, CPSD, HEL-S-130P, Cathepsin D, CTSD
Background:This gene encodes a member of the A1 family of peptidases. The encoded preproprotein is proteolytically processed to generate multiple protein products. These products include the cathepsin D light and heavy chains, which heterodimerize to form the mature enzyme. This enzyme exhibits pepsin-like activity and plays a role in protein turnover and in the proteolytic activation of hormones and growth factors. Mutations in this gene play a causal role in neuronal ceroid lipofuscinosis-10 and may be involved in the pathogenesis of several other diseases, including breast cancer and possibly Alzheimer's disease.
UniProt Protein Function:CTSD: Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease. Consists of a light chain and a heavy chain. Belongs to the peptidase A1 family.
UniProt Protein Details:

Protein type:Autophagy; Motility/polarity/chemotaxis; Protease; EC 3.4.23.5

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: extracellular matrix; extracellular space; lysosomal lumen; lysosome; melanosome; extracellular region

Molecular Function:protein binding; aspartic-type endopeptidase activity

Biological Process: collagen catabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; antigen processing and presentation of exogenous peptide antigen via MHC class II; proteolysis

Disease: Ceroid Lipofuscinosis, Neuronal, 10

NCBI Summary:This gene encodes a lysosomal aspartyl protease composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. This proteinase, which is a member of the peptidase C1 family, has a specificity similar to but narrower than that of pepsin A. Transcription of this gene is initiated from several sites, including one which is a start site for an estrogen-regulated transcript. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease. [provided by RefSeq, Jul 2008]
UniProt Code:P07339
NCBI GenInfo Identifier:115717
NCBI Gene ID:1509
NCBI Accession:P07339.1
UniProt Secondary Accession:P07339,Q6IB57,
UniProt Related Accession:P07339
Molecular Weight:44,552 Da
NCBI Full Name:Cathepsin D
NCBI Synonym Full Names:cathepsin D
NCBI Official Symbol:CTSD  
NCBI Official Synonym Symbols:CPSD; CLN10; HEL-S-130P  
NCBI Protein Information:cathepsin D; lysosomal aspartyl protease; lysosomal aspartyl peptidase; ceroid-lipofuscinosis, neuronal 10; epididymis secretory sperm binding protein Li 130P
UniProt Protein Name:Cathepsin D
Protein Family:Cathepsin
UniProt Gene Name:CTSD  
UniProt Entry Name:CATD_HUMAN
Anti-CTSD Antibody (CAB13292)Western blot analysis of extracts of various cell lines, using CTSD antibody (CAB13292) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-CTSD Antibody (CAB13292)Western blot analysis of extracts of various cell lines, using CTSD antibody (CAB13292) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
Anti-CTSD Antibody (CAB13292)Immunohistochemistry of paraffin-embedded rat brain using CTSD Rabbit pAb (CAB13292) at dilution of 1:100 (40x lens).
Anti-CTSD Antibody (CAB13292)Immunohistochemistry of paraffin-embedded rat spleen using CTSD Rabbit pAb (CAB13292) at dilution of 1:100 (40x lens).
Anti-CTSD Antibody (CAB13292)Immunohistochemistry of paraffin-embedded human liver using CTSD Rabbit pAb (CAB13292) at dilution of 1:100 (40x lens).
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