Cell Biology Antibodies 9

Anti-CTSA Antibody (CAB5503)

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SKU:
CAB5503
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-CTSA Antibody
Antibody SKU:CAB5503
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 210-450 of human CTSA (NP_001121167.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:20 - 1:50
Reactivity:Human, Mouse, Rat
Positive Samples:SH-SY5Y, Mouse brain, Rat kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 210-450 of human CTSA (NP_001121167.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SYEQ NDNS LVYF AYYH GLLG NRLW SSLQ THCC SQNK CNFY DNKD LECV TNLQ EVAR IVGN SGLN IYNL YAPC AGGV PSHF RYEK DTVV VQDL GNIF TRLP LKRM WHQA LLRS GDKV RMDP PCTN TTAA STYL NNPY VRKA LNIP EQLP QWDM CNFL VNLQ YRRL YRSM NSQY LKLL SSQK YQIL LYNG DVDM ACNF MGDE WFVD SLNQ KMEV QRRP WLVK YGDS GEQI AGFV KEFS HIAF L
Gene ID:5476
Uniprot:P10619
Cellular Location:Lysosome
Calculated MW:52kDa/54kDa
Observed MW:54KDa
Synonyms:CTSA, GLB2, GSL, NGBE, PPCA, PPGB
Background:This gene encodes a member of the peptidase S10 family of serine carboxypeptidases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate two chains that comprise the heterodimeric active enzyme. This enzyme possesses deamidase, esterase and carboxypeptidase activities and acts as a scaffold in the lysosomal multienzyme complex. Mutations in this gene are associated with galactosialidosis.
UniProt Protein Function:CTSA: Protective protein appears to be essential for both the activity of beta-galactosidase and neuraminidase, it associates with these enzymes and exerts a protective function necessary for their stability and activity. This protein is also a carboxypeptidase and can deamidate tachykinins. Defects in CTSA are the cause of galactosialidosis (GSL). A lysosomal storage disease associated with a combined deficiency of beta-galactosidase and neuraminidase, secondary to a defect in cathepsin A. All patients have clinical manifestations typical of a lysosomal disorder, such as coarse facies, cherry red spots, vertebral changes, foam cells in the bone marrow, and vacuolated lymphocytes. Three phenotypic subtypes are recognized. The early infantile form is associated with fetal hydrops, edema, ascites, visceromegaly, skeletal dysplasia, and early death. The late infantile type is characterized by hepatosplenomegaly, growth retardation, cardiac involvement, and a normal or mildly affected mental state. The juvenile/adult form is characterized by myoclonus, ataxia, angiokeratoma, mental retardation, neurologic deterioration, absence of visceromegaly, and long survival. Belongs to the peptidase S10 family.
UniProt Protein Details:

Protein type:Mitochondrial; EC 3.4.16.5; Protease; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 20q13.1

Cellular Component: nucleoplasm; lysosomal lumen; intracellular membrane-bound organelle; membrane; lysosome; endoplasmic reticulum

Molecular Function:serine carboxypeptidase activity; carboxypeptidase activity; enzyme activator activity

Biological Process: positive regulation of catalytic activity; intracellular protein transport; cellular protein metabolic process; sphingolipid metabolic process; dolichol-linked oligosaccharide biosynthetic process; glycosphingolipid metabolic process; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification; proteolysis

Disease: Galactosialidosis

NCBI Summary:This gene encodes a glycoprotein which associates with lysosomal enzymes beta-galactosidase and neuraminidase to form a complex of high molecular weight multimers. The formation of this complex provides a protective role for stability and activity. Deficiencies in this gene are linked to multiple forms of galactosialidosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:P10619
NCBI GenInfo Identifier:20178316
NCBI Gene ID:5476
NCBI Accession:P10619.2
UniProt Secondary Accession:P10619,Q561W6, Q5JZH1, Q96KJ2, Q9BR08, Q9BW68, B2R798
UniProt Related Accession:P10619
Molecular Weight:480
NCBI Full Name:Lysosomal protective protein
NCBI Synonym Full Names:cathepsin A
NCBI Official Symbol:CTSA  
NCBI Official Synonym Symbols:GSL; GLB2; NGBE; PPCA; PPGB  
NCBI Protein Information:lysosomal protective protein; deamidase; urinary kininase; carboxypeptidase C; carboxypeptidase L; carboxypeptidase-L; beta-galactosidase 2; lysosomal carboxypeptidase A; protective protein cathepsin A; carboxypeptidase Y-like kininase; beta-galactosidase protective protein
UniProt Protein Name:Lysosomal protective protein
UniProt Synonym Protein Names:Carboxypeptidase C; Carboxypeptidase L; Cathepsin A; Protective protein cathepsin A; PPCA; Protective protein for beta-galactosidaseCleaved into the following 2 chains:Lysosomal protective protein 32 kDa chain; Lysosomal protective protein 20 kDa chain
Protein Family:Lysosomal protective protein
UniProt Gene Name:CTSA  
UniProt Entry Name:PPGB_HUMAN
Anti-CTSA Antibody (CAB5503)Western blot analysis of extracts of various cell lines, using CTSA antibody (CAB5503) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
Anti-CTSA Antibody (CAB5503)Immunofluorescence analysis of MCF-7 cells using CTSA antibody (CAB5503). Blue: DAPI for nuclear staining.
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