Name: Anti-CTR9 Antibody (CAB3659)
Brand: Epigenetics & Nuclear Signaling Antibodies 3
SKU: CAB3659
Availability: OutOfStock

Antibody Name:	Anti-CTR9 Antibody
Antibody SKU:	CAB3659
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1024-1173 of human CTR9 (NP_055448.1).

Application:	WB
Recommended Dilution:	WB 1:1000 - 1:2000
Reactivity:	Human
Positive Samples:	HeLa, Jurkat

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1024-1173 of human CTR9 (NP_055448.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	DKLK IADE GHPR NSNS NSDS DEDE QRKK CASS ESDS DENQ NKSG SEAG SPRR PRRQ RSDQ DSDS DQPS RKRR PSGS EQSD NESV QSGR SHSG VSEN DSRP ASPS AESD HESE RGSD NEGS GQGS GNES EPEG SNNE ASDR GSEH GSDD SD
Gene ID:	9646
Uniprot:	Q6PD62
Cellular Location:	Nucleus speckle
Calculated MW:	133kDa
Observed MW:	145kDa

Synonyms:	CTR9, SH2BP1, TSBP, p150, p150TSP
Background:	The protein encoded by this gene is a component of the PAF1 complex, which associates with RNA polymerase II and functions in transcriptional regulation and elongation. This complex also plays a role in the modification of histones.

UniProt Protein Function:	SH2BP1: Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non- phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of MLL1; it promotes leukemogenesis though association with MLL-rearranged oncoproteins, such as MLL-MLLT3/AF9 and MLL-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Required for mono- and trimethylation on histone H3 'Lys-4' (H3K4me3) and dimethylation on histone H3 'Lys-79' (H3K4me3). Required for Hox gene transcription. Required for the trimethylation of histone H3 'Lys-4' (H3K4me3) on genes involved in stem cell pluripotency; this function is synergistic with CXXC1 indicative for an involvement of the SET1 complex. Involved in transcriptional regulation of IL6-responsive genes and in JAK-STAT pathway; may regulate DNA-association of STAT3.
UniProt Protein Details:	Protein type:Transcription regulation Chromosomal Location of Human Ortholog: 11p15.3 Cellular Component: nuclear speck Molecular Function:protein binding; SH2 domain binding Biological Process: histone monoubiquitination; negative regulation of myeloid cell differentiation; Wnt receptor signaling pathway; transcription, DNA-dependent; endodermal cell fate commitment; positive regulation of RNA elongation from RNA polymerase II promoter; histone H2B ubiquitination; stem cell maintenance; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; positive regulation of histone H3-K4 methylation; JAK-STAT cascade
NCBI Summary:	The protein encoded by this gene is a component of the PAF1 complex, which associates with RNA polymerase II and functions in transcriptional regulation and elongation. This complex also plays a role in the modification of histones. [provided by RefSeq, Oct 2016]
UniProt Code:	Q6PD62
NCBI GenInfo Identifier:	74758318
NCBI Gene ID:	9646
NCBI Accession:	Q6PD62.1
UniProt Related Accession:	Q6PD62
Molecular Weight:	134kDa
NCBI Full Name:	RNA polymerase-associated protein CTR9 homolog
NCBI Synonym Full Names:	CTR9 homolog, Paf1/RNA polymerase II complex component
NCBI Official Symbol:	CTR9
NCBI Official Synonym Symbols:	TSBP; p150; SH2BP1; p150TSP
NCBI Protein Information:	RNA polymerase-associated protein CTR9 homolog
UniProt Protein Name:	RNA polymerase-associated protein CTR9 homolog
UniProt Synonym Protein Names:	SH2 domain-binding protein 1
Protein Family:	RNA polymerase-associated protein
UniProt Gene Name:	CTR9
UniProt Entry Name:	CTR9_HUMAN

Western blot analysis of extracts of various cell lines, using CTR9 antibody (CAB3659) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

Antibodies

Anti-CTR9 Antibody (CAB16471)

CTR9 Antibody (PACO08666)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)