Metabolism Antibodies 2

Anti-CTH Antibody (CAB6121)

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SKU:
CAB6121
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-CTH Antibody
Antibody SKU:CAB6121
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-405 of human CTH (NP_001893.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:HepG2, HeLa, Mouse liver, Mouse kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-405 of human CTH (NP_001893.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MQEK DASS QGFL PHFQ HFAT QAIH VGQD PEQW TSRA VVPP ISLS TTFK QGAP GQHS GFEY SRSG NPTR NCLE KAVA ALDG AKYC LAFA SGLA ATVT ITHL LKAG DQII CMDD VYGG TNRY FRQV ASEF GLKI SFVD CSKI KLLE AAIT PETK LVWI ETPT NPTQ KVID IEGC AHIV HKHG DIIL VVDN TFMS PYFQ RPLA LGAD ISMY SATK YMNG HSDV VMGL VSVN CESL HNRL RFLQ NSLG AVPS PIDC YLCN RGLK TLHV RMEK HFKN GMAV AQFL ESNP WVEK VIYP GLPS HPQH ELVK RQCT GCTG MVTF YIKG TLQH AEIF LKNL KLFT LAES LGGF ESLA ELPA IMTH ASVL KNDR DVLG ISDT LIRL SVGL EDEE DLLE DLDQ ALKA AHPP SGSH S
Gene ID:1491
Uniprot:P32929
Cellular Location:Cytoplasm
Calculated MW:39kDa/41kDa/44kDa
Observed MW:45kDa
Synonyms:CTH
Background:This gene encodes a cytoplasmic enzyme in the trans-sulfuration pathway that converts cystathione derived from methionine into cysteine. Glutathione synthesis in the liver is dependent upon the availability of cysteine. Mutations in this gene cause cystathioninuria. Alternative splicing of this gene results in three transcript variants encoding different isoforms.
UniProt Protein Function:CTH: Catalyzes the last step in the trans-sulfuration pathway from methionine to cysteine. Has broad substrate specificity. Converts cystathionine to cysteine, ammonia and 2-oxobutanoate. Converts two cysteine molecules to lanthionine and hydrogen sulfide. Can also accept homocysteine as substrate. Specificity depends on the levels of the endogenous substrates. Generates the endogenous signaling molecule hydrogen sulfide (H2S), and so contributes to the regulation of blood pressure. Acts as a cysteine-protein sulfhydrase by mediating sulfhydration of target proteins: sulfhydration consists of converting -SH groups into -SSH on specific cysteine residues of target proteins such as GAPDH, PTPN1 and NF-kappa-B subunit RELA, thereby regulating their function. Defects in CTH are the cause of cystathioninuria (CSTNU). It is an autosomal recessive phenotype characterized by abnormal accumulation of plasma cystathionine, leading to increased urinary excretion. Belongs to the trans-sulfuration enzymes family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 4.4.1.1; Amino Acid Metabolism - cysteine and methionine; Lyase; Energy Metabolism - nitrogen; Cell cycle regulation; Amino Acid Metabolism - glycine, serine and threonine; Other Amino Acids Metabolism - selenoamino acid

Chromosomal Location of Human Ortholog: 1p31.1

Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus

Molecular Function:calmodulin binding; protein binding; cystathionine gamma-lyase activity; homocysteine desulfhydrase activity; carbon-sulfur lyase activity; pyridoxal phosphate binding

Biological Process: cysteine metabolic process; positive regulation of I-kappaB kinase/NF-kappaB cascade; sulfur amino acid metabolic process; unfolded protein response; sulfur amino acid catabolic process; protein-pyridoxal-5-phosphate linkage via peptidyl-N6-pyridoxal phosphate-L-lysine; transsulfuration; cysteine biosynthetic process; activation of NF-kappaB transcription factor; protein homotetramerization

Disease: Cystathioninuria

NCBI Summary:This gene encodes a cytoplasmic enzyme in the trans-sulfuration pathway that converts cystathione derived from methionine into cysteine. Glutathione synthesis in the liver is dependent upon the availability of cysteine. Mutations in this gene cause cystathioninuria. Alternative splicing of this gene results in three transcript variants encoding different isoforms. [provided by RefSeq, Jun 2010]
UniProt Code:P32929
NCBI GenInfo Identifier:27735163
NCBI Gene ID:1491
NCBI Accession:P32929.3
UniProt Secondary Accession:P32929,Q53FB3, Q53Y79, Q9H4W7, Q9H4W8, B4E1R2, E9PDV0
UniProt Related Accession:P32929
Molecular Weight:41,260 Da
NCBI Full Name:Cystathionine gamma-lyase
NCBI Synonym Full Names:cystathionine gamma-lyase
NCBI Official Symbol:CTH  
NCBI Protein Information:cystathionine gamma-lyase; gamma-cystathionase; homoserine deaminase; cysteine desulfhydrase; homoserine dehydratase; cysteine-protein sulfhydrase; cystathionase (cystathionine gamma-lyase)
UniProt Protein Name:Cystathionine gamma-lyase
UniProt Synonym Protein Names:Cysteine-protein sulfhydrase; Gamma-cystathionase
Protein Family:Cystathionine gamma-lyase
UniProt Gene Name:CTH  
UniProt Entry Name:CGL_HUMAN
Anti-CTH Antibody (CAB6121)Western blot analysis of extracts of various cell lines, using CTH antibody (CAB6121) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
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