Antibody Name:	Anti-CST1 Antibody
Antibody SKU:	CAB13290
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 20-141 of human CST1 (NP_001889.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human
Positive Samples:	SW480, BT-474, THP-1

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 20-141 of human CST1 (NP_001889.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	AWSP KEED RIIP GGIY NADL NDEW VQRA LHFA ISEY NKAT KDDY YRRP LRVL RARQ QTVG GVNY FFDV EVGR TICT KSQP NLDT CAFH EQPE LQKK QLCS FEIY EVPW ENRR SLVK SRCQ ES
Gene ID:	1469
Uniprot:	P01037
Cellular Location:	Secreted
Calculated MW:	16kDa
Observed MW:	16kDa

Synonyms:

CST1

Background:

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes a cysteine proteinase inhibitor found in saliva, tears, urine, and seminal fluid.

UniProt Protein Function:	CST1: Human saliva appears to contain several cysteine proteinase inhibitors that are immunologically related to cystatin S but that differ in their specificity due to amino acid sequence differences. Cystatin SN, with a pI of 7.5, is a much better inhibitor of papain and dipeptidyl peptidase I than is cystatin S, although both inhibit ficin equally well. Belongs to the cystatin family.
UniProt Protein Details:	Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 20p11.21 Cellular Component: extracellular space Molecular Function:cysteine protease inhibitor activity Biological Process: detection of chemical stimulus involved in sensory perception of bitter taste
NCBI Summary:	The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes a cysteine proteinase inhibitor found in saliva, tears, urine, and seminal fluid. [provided by RefSeq, Jul 2008]
UniProt Code:	P01037
NCBI GenInfo Identifier:	311033460
NCBI Gene ID:	1469
NCBI Accession:	P01037.3
UniProt Secondary Accession:	P01037,Q96LE6, Q9UCQ6,
UniProt Related Accession:	P01037
Molecular Weight:	141
NCBI Full Name:	Cystatin-SN
NCBI Synonym Full Names:	cystatin SN
NCBI Official Symbol:	CST1
NCBI Protein Information:	cystatin-SN; cystatin 1; cystatin-1; cystain-SA-I; cystatin SA-I; salivary cystatin-SA-1; cysteine proteinase inhibitor, type 2 family
UniProt Protein Name:	Cystatin-SN
UniProt Synonym Protein Names:	Cystain-SA-I; Cystatin-1; Salivary cystatin-SA-1
Protein Family:	Cystatin
UniProt Gene Name:	CST1
UniProt Entry Name:	CYTN_HUMAN

	Western blot analysis of extracts of various cell lines, using CST1 Antibody (CAB13290) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of A549 cells using CST1 antibody (CAB13290). Blue: DAPI for nuclear staining.