Signal Transduction Antibodies 2

Anti-CRYBA4 Antibody (CAB15268)

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SKU:
CAB15268
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-CRYBA4 Antibody
Antibody SKU:CAB15268
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-196 of human CRYBA4 (NP_001877.1).
Application:WB
Recommended Dilution:WB 1:200 - 1:2000
Reactivity:Human
Positive Samples:293T, HeLa
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-196 of human CRYBA4 (NP_001877.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MTLQ CTKS AGPW KMVV WDED GFQG RRHE FTAE CPSV LELG FETV RSLK VLSG AWVG FEHA GFQG QQYI LERG EYPS WDAW GGNT AYPA ERLT SFRP AACA NHRD SRLT IFEQ ENFL GKKG ELSD DYPS LQAM GWEG NEVG SFHV HSGA WVCS QFPG YRGF QYVL ECDH HSGD YKHF REWG SHAP TFQV QSIR RIQQ
Gene ID:1413
Uniprot:P53673
Cellular Location:
Calculated MW:22kDa
Observed MW:22kDa
Synonyms:CRYBA4, CTRCT23, CYRBA4, MCOPCT4
Background:Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Beta-crystallins, the most heterogeneous, differ by the presence of the C-terminal extension (present in the basic group, none in the acidic group). Beta-crystallins form aggregates of different sizes and are able to self-associate to form dimers or to form heterodimers with other beta-crystallins. This gene, a beta acidic group member, is part of a gene cluster with beta-B1, beta-B2, and beta-B3.
UniProt Protein Function:CRYBA4: a major structural protein of the eye lens. A member of the beta/gamma-crystallin family.
UniProt Protein Details:

Chromosomal Location of Human Ortholog: 22q12.1

Molecular Function:protein binding

Biological Process: camera-type eye development; visual perception

Disease: Cataract 23

NCBI Summary:Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Beta-crystallins, the most heterogeneous, differ by the presence of the C-terminal extension (present in the basic group, none in the acidic group). Beta-crystallins form aggregates of different sizes and are able to self-associate to form dimers or to form heterodimers with other beta-crystallins. This gene, a beta acidic group member, is part of a gene cluster with beta-B1, beta-B2, and beta-B3. [provided by RefSeq, Jul 2008]
UniProt Code:P53673
NCBI GenInfo Identifier:2506318
NCBI Gene ID:1413
NCBI Accession:P53673.3
UniProt Secondary Accession:P53673,Q4VB22, Q6ICE4,
UniProt Related Accession:P53673
Molecular Weight:22,374 Da
NCBI Full Name:Beta-crystallin A4
NCBI Synonym Full Names:crystallin beta A4
NCBI Official Symbol:CRYBA4  
NCBI Official Synonym Symbols:CYRBA4; CTRCT23; MCOPCT4  
NCBI Protein Information:beta-crystallin A4
UniProt Protein Name:Beta-crystallin A4
UniProt Synonym Protein Names:Beta-A4 crystallin
Protein Family:Beta-crystallin
UniProt Gene Name:CRYBA4  
UniProt Entry Name:CRBA4_HUMAN
Anti-CRYBA4 Antibody (CAB15268)Western blot analysis of extracts of various cell lines, using CRYBA4 antibody (CAB15268) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30S.
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