Neuroscience

Anti-CRYBA1 Antibody (CAB15267)

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SKU:
CAB15267
Product Type:
Antibody
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Neuroscience

Description

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Antibody Name:Anti-CRYBA1 Antibody
Antibody SKU:CAB15267
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-215 of human CRYBA1 (NP_005199.2).
Application:WB
Recommended Dilution:WB 1:200 - 1:2000
Reactivity:Mouse, Rat
Positive Samples:Mouse eye, Rat eye
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-215 of human CRYBA1 (NP_005199.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:METQ AEQQ ELET LPTT KMAQ TNPT PGSL GPWK ITIY DQEN FQGK RMEF TSSC PNVS ERSF DNVR SLKV ESGA WIGY EHTS FCGQ QFIL ERGE YPRW DAWS GSNA YHIE RLMS FRPI CSAN HKES KMTI FEKE NFIG RQWE ISDD YPSL QAMG WFNN EVGS MKIQ SGAW VCYQ YPGY RGYQ YILE CDHH GGDY KHWR EWGS HAQT SQIQ SIRR IQQ
Gene ID:1411
Uniprot:P05813
Cellular Location:
Calculated MW:23kDa/25kDa
Observed MW:25kDa
Synonyms:CRYBA1, CRYB1, CTRCT10
Background:Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Beta-crystallins, the most heterogeneous, differ by the presence of the C-terminal extension (present in the basic group, none in the acidic group). Beta-crystallins form aggregates of different sizes and are able to self-associate to form dimers or to form heterodimers with other beta-crystallins. This gene, a beta acidic group member, encodes two proteins (crystallin, beta A3 and crystallin, beta A1) from a single mRNA, the latter protein is 17 aa shorter than crystallin, beta A3 and is generated by use of an alternate translation initiation site. Deletion of exons 3 and 4 causes the autosomal dominant disease 'zonular cataract with sutural opacities'.
UniProt Protein Function:CRYBA1: a major structural protein of the eye lens. A member of the beta/gamma-crystallin family. Defects are the cause of congenital zonular cataract with sutural opacities. Two splice-variant isoforms have been described.
UniProt Protein Details:

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: cytoplasm; nucleus

Molecular Function:protein binding

Biological Process: visual perception

Disease: Cataract 10, Multiple Types

NCBI Summary:Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Beta-crystallins, the most heterogeneous, differ by the presence of the C-terminal extension (present in the basic group, none in the acidic group). Beta-crystallins form aggregates of different sizes and are able to self-associate to form dimers or to form heterodimers with other beta-crystallins. This gene, a beta acidic group member, encodes two proteins (crystallin, beta A3 and crystallin, beta A1) from a single mRNA, the latter protein is 17 aa shorter than crystallin, beta A3 and is generated by use of an alternate translation initiation site. Deletion of exons 3 and 4 causes the autosomal dominant disease 'zonular cataract with sutural opacities'. [provided by RefSeq, Jul 2008]
UniProt Code:P05813
NCBI GenInfo Identifier:2506317
NCBI Gene ID:1411
NCBI Accession:P05813.4
UniProt Secondary Accession:P05813,Q13633, Q14CM9,
UniProt Related Accession:P05813
Molecular Weight:23,191 Da
NCBI Full Name:Beta-crystallin A3
NCBI Synonym Full Names:crystallin beta A1
NCBI Official Symbol:CRYBA1  
NCBI Official Synonym Symbols:CRYB1; CTRCT10  
NCBI Protein Information:beta-crystallin A3
UniProt Protein Name:Beta-crystallin A3
Protein Family:Beta-crystallin
UniProt Gene Name:CRYBA1  
UniProt Entry Name:CRBA1_HUMAN
Anti-CRYBA1 Antibody (CAB15267)Western blot analysis of extracts of various cell lines, using CRYBA1 antibody (CAB15267) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
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