Signal Transduction Antibodies 3
Anti-CRYAA Antibody (CAB5725)
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- SKU:
- CAB5725
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Reactivity:
- Fish - Zebrafish
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Description
| Antibody Name: | Anti-CRYAA Antibody |
| Antibody SKU: | CAB5725 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat, Zebrafish |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-173 of human CRYAA (NP_000385.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat, Zebrafish |
| Positive Samples: | Mouse kidney, Mouse liver, Mouse heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-173 of human CRYAA (NP_000385.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MDVT IQHP WFKR TLGP FYPS RLFD QFFG EGLF EYDL LPFL SSTI SPYY RQSL FRTV LDSG ISEV RSDR DKFV IFLD VKHF SPED LTVK VQDD FVEI HGKH NERQ DDHG YISR EFHR RYRL PSNV DQSA LSCS LSAD GMLT FCGP KIQT GLDA THAE RAIP VSRE EKPT SAPS S |
| Gene ID: | 1409 |
| Uniprot: | P02489 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 19kDa |
| Observed MW: | 23kDa |
| Synonyms: | CRYAA, CRYA1, CTRCT9, HSPB4 |
| Background: | Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Defects in this gene cause autosomal dominant congenital cataract (ADCC). |
| UniProt Protein Function: | CRYA1: a major structural protein of the eye lens. A member of the small heat shock protein (sHSP, also known as the HSP20) family. Alpha-A is preferentially restricted to the lens. |
| UniProt Protein Details: | Protein type:Heat shock protein; Chaperone Chromosomal Location of Human Ortholog: 21q22.3 Cellular Component: cytoplasm; nucleus Molecular Function:identical protein binding; protein binding; metal ion binding; unfolded protein binding; structural constituent of eye lens Biological Process: negative regulation of intracellular transport; visual perception; response to stimulus; protein refolding; protein homooligomerization; negative regulation of apoptosis Disease: Cataract 9, Multiple Types |
| NCBI Summary: | Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Defects in this gene cause autosomal dominant congenital cataract (ADCC). [provided by RefSeq, Jan 2014] |
| UniProt Code: | P02489 |
| NCBI GenInfo Identifier: | 1706112 |
| NCBI Gene ID: | 1409 |
| NCBI Accession: | P02489.2 |
| UniProt Secondary Accession: | P02489,Q53X53, |
| UniProt Related Accession: | P02489,AAB33370 |
| Molecular Weight: | 173 |
| NCBI Full Name: | Alpha-crystallin A chain |
| NCBI Synonym Full Names: | crystallin, alpha A |
| NCBI Official Symbol: | CRYAA |
| NCBI Official Synonym Symbols: | CRYA1; HSPB4; CTRCT9 |
| NCBI Protein Information: | alpha-crystallin A chain; crystallin, alpha-1; heat shock protein beta-4; human alphaA-crystallin (CRYA1) |
| UniProt Protein Name: | Alpha-crystallin A chain |
| UniProt Synonym Protein Names: | Heat shock protein beta-4; HspB4 |
| Protein Family: | Alpha-crystallin |
| UniProt Gene Name: | CRYAA |
| UniProt Entry Name: | CRYAA_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CRYAA antibody (CAB5725) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunohistochemistry of paraffin-embedded rat kidney using CRYAA antibody (CAB5725) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human colon carcinoma using CRYAA antibody (CAB5725) at dilution of 1:200 (40x lens). |
 | Immunofluorescence analysis of HeLa cells using CRYAA antibody (CAB5725). Blue: DAPI for nuclear staining. |
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