Metabolism Antibodies 2

Anti-CPT1A Antibody (CAB5307)

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SKU:
CAB5307
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism

Description

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Antibody Name:Anti-CPT1A Antibody
Antibody SKU:CAB5307
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 497-756 of human CPT1A (NP_001027017.1).
Application:WB IHC IP
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IP 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:MCF7, HepG2, Mouse heart, Rat heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 497-756 of human CPT1A (NP_001027017.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:GYAE DGHC KGDI NPNI PYPT RLQW DIPG ECQE VIET SLNT ANLL ANDV DFHS FPFV AFGK GIIK KCRT SPDA FVQL ALQL AHYK DMGK FCLT YEAS MTRL FREG RTET VRSC TTES CDFV RAMV DPAQ TVEQ RLKL FKLA SEKH QHMY RLAM TGSG IDRH LFCL YVVS KYLA VESP FLKE VLSE PWRL STSQ TPQQ QVEL FDLE NNPE YVSS GGGF GPVA DDGY GVSY ILVG ENLI NFHI SSKF SCPE TGII SQGP SSDT
Gene ID:1374
Uniprot:P50416
Cellular Location:Mitochondrion outer membrane, Multi-pass membrane protein
Calculated MW:86kDa/88kDa
Observed MW:88kDa
Synonyms:CPT1A, CPT1, CPT1-L, L-CPT1
Background:The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:CPT1A: Belongs to the carnitine/choline acetyltransferase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Lipid Metabolism - fatty acid; Membrane protein, integral; EC 2.3.1.21; Mitochondrial; Membrane protein, multi-pass; Transferase

Chromosomal Location of Human Ortholog: 11q13.2

Cellular Component: mitochondrial outer membrane; membrane; mitochondrion; mitochondrial inner membrane; integral to mitochondrial outer membrane

Molecular Function:identical protein binding; carnitine O-palmitoyltransferase activity

Biological Process: circadian rhythm; response to drug; eating behavior; glucose metabolic process; long-chain fatty acid metabolic process; carnitine shuttle; cellular lipid metabolic process; response to organic cyclic substance; positive regulation of fatty acid beta-oxidation; fatty acid beta-oxidation; triacylglycerol metabolic process; epithelial cell differentiation; carnitine metabolic process; regulation of insulin secretion; protein homooligomerization

Disease: Carnitine Palmitoyltransferase I Deficiency

NCBI Summary:The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:P50416
NCBI GenInfo Identifier:56405343
NCBI Gene ID:1374
NCBI Accession:P50416.2
UniProt Secondary Accession:P50416,Q8TCU0, Q9BWK0,
UniProt Related Accession:P50416
Molecular Weight:773
NCBI Full Name:Carnitine O-palmitoyltransferase 1, liver isoform
NCBI Synonym Full Names:carnitine palmitoyltransferase 1A (liver)
NCBI Official Symbol:CPT1A  
NCBI Official Synonym Symbols:CPT1; CPT1-L; L-CPT1  
NCBI Protein Information:carnitine O-palmitoyltransferase 1, liver isoform; CPT I; CPTI-L; carnitine palmitoyltransferase I, liver; carnitine O-palmitoyltransferase I, liver isoform
UniProt Protein Name:Carnitine O-palmitoyltransferase 1, liver isoform
UniProt Synonym Protein Names:Carnitine O-palmitoyltransferase I, liver isoform; CPT I; CPTI-L; Carnitine palmitoyltransferase 1A
Protein Family:Carnitine O-palmitoyltransferase
UniProt Gene Name:CPT1A  
UniProt Entry Name:CPT1A_HUMAN
Anti-CPT1A Antibody (CAB5307)Western blot analysis of extracts of various cell lines, using CPT1A antibody (CAB5307) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-CPT1A Antibody (CAB5307)Immunohistochemistry of paraffin-embedded Human colon carcinoma using CPT1A Rabbit pAb (CAB5307) at dilution of 1:100 (40x lens).
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