Anti-CPSF73 Antibody (CAB2222)

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SKU:
CAB2222
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
CPSF3
Synonyms:
CPSF-73
Synonyms:
CPSF73
Synonyms:
cleavage and polyadenylation specific factor 3
Research Area:
Epigenetics and Nuclear Signaling

Description

Product Name:CPSF73 Rabbit mAb
Product Code:CAB2222
Size:20uL, 50uL, 100uL
Synonyms:CPSF3, CPSF-73, CPSF73, cleavage and polyadenylation specific factor 3
Applications:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthesized peptide derived from human CPSF73.
Applications:WB
Recommended Dilutions:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, 293T, SH-SY5Y, Mouse liver, Mouse kidney, Mouse uterus, Rat spleen
Immunogen:A synthesized peptide derived from human CPSF73.
Purification Method:Affinity purification
Storage:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:51692
Uniprot:Q9UKF6
Cellular Location:Nucleus
Calculated MW:77kDa
Observed MW:77KDa
UniProt Protein Function:CPSF3: Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Has endonuclease activity, and functions as mRNA 3'-end-processing endonuclease. Also involved in the histone 3'-end pre-mRNA processing. U7 snRNP- dependent protein that induces both the 3'-endoribonucleolytic cleavage of histone pre-mRNAs and acts as a 5' to 3' exonuclease for degrading the subsequent downstream cleavage product (DCP) of mature histone mRNAs. Cleavage occurs after the 5'-ACCCA-3' sequence in the histone pre-mRNA leaving a 3'hydroxyl group on the upstream fragment containing the stem loop (SL) and 5' phosphate on the downstream cleavage product (DCP) starting with CU nucleotides. The U7-dependent 5' to 3' exonuclease activity is processive and degrades the DCP RNA substrate even after complete removal of the U7-binding site. Binds to the downstream cleavage product (DCP) of histone pre-mRNAs and the cleaved DCP RNA substrate in a U7 snRNP dependent manner. Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. CPSF3 subfamily.
UniProt Protein Details:

Protein type:EC 3.1.27.-; RNA processing; RNA splicing; RNA-binding; Ribonuclease

Chromosomal Location of Human Ortholog: 2p25.1

Cellular Component: mRNA cleavage and polyadenylation specificity factor complex; nucleoplasm

Molecular Function:5'-3' exonuclease activity; endoribonuclease activity; protein binding; RNA binding

Biological Process: histone mRNA 3'-end processing; mRNA 3'-end processing; mRNA cleavage; mRNA export from nucleus; mRNA polyadenylation; nuclear mRNA splicing, via spliceosome; termination of RNA polymerase II transcription

NCBI Summary:This gene encodes a member of the metallo-beta-lactamase family. The encoded protein is a 73kDa subunit of the cleavage and polyadenylation specificity factor and functions as an endonuclease that recognizes the pre-mRNA 3'-cleavage site AAUAAA prior to polyadenylation. It also cleaves after the pre-mRNA sequence ACCCA during histone 3'-end pre-mRNA processing. [provided by RefSeq, Oct 2012]
UniProt Code:Q9UKF6
NCBI GenInfo Identifier:18203503
NCBI Gene ID:51692
NCBI Accession:Q9UKF6.1
UniProt Secondary Accession:Q9UKF6,O14769, Q53RS2, Q96F36,
UniProt Related Accession:Q9UKF6
Molecular Weight:77kDa
NCBI Full Name:Cleavage and polyadenylation specificity factor subunit 3
NCBI Synonym Full Names:cleavage and polyadenylation specific factor 3
NCBI Official Symbol:CPSF3  
NCBI Official Synonym Symbols:CPSF73; CPSF-73  
NCBI Protein Information:cleavage and polyadenylation specificity factor subunit 3
UniProt Protein Name:Cleavage and polyadenylation specificity factor subunit 3
UniProt Synonym Protein Names:Cleavage and polyadenylation specificity factor 73 kDa subunit; CPSF 73 kDa subunit; mRNA 3'-end-processing endonuclease CPSF-73
Protein Family:Cleavage and polyadenylation specificity factor
UniProt Gene Name:CPSF3  
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