Name: Anti-CPNE1 Antibody (CAB15749)
Brand: Developmental Biology
SKU: CAB15749
Availability: OutOfStock

Antibody Name:	Anti-CPNE1 Antibody
Antibody SKU:	CAB15749
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 120-220 of human CPNE1 (NP_003906.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Mouse, Rat
Positive Samples:	Mouse brain, Mouse heart, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 120-220 of human CPNE1 (NP_003906.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MLKP GKPA GRGT ITVS AQEL KDNR VVTM EVEA RNLD KKDF LGKS DPFL EFFR QGDG KWHL VYRS EVIK NNLN PTWK RFSV PVQH FCGG NPST PIQV QCSD Y
Gene ID:	8904
Uniprot:	Q99829
Cellular Location:	Cell membrane, Cytoplasm, Nucleus
Calculated MW:	59kDa
Observed MW:	65kDa

Synonyms:

CPNE1, COPN1, CPN1, copine-1

Background:

Calcium-dependent membrane-binding proteins may regulate molecular events at the interface of the cell membrane and cytoplasm. This gene encodes a calcium-dependent protein that also contains two N-terminal type II C2 domains and an integrin A domain-like sequence in the C-terminus. However, the encoded protein does not contain a predicted signal sequence or transmembrane domains. This protein has a broad tissue distribution and it may function in membrane trafficking. This gene and the gene for RNA binding motif protein 12 overlap at map location 20q11.21. Alternate splicing results in multiple transcript variants encoding different proteins.

UniProt Protein Function:	Calcium-dependent phospholipid-binding protein that plays a role in calcium-mediated intracellular processes (PubMed:14674885). Involved in the TNF-alpha receptor signaling pathway in a calcium-dependent manner (PubMed:14674885). Exhibits calcium-dependent phospholipid binding properties (PubMed:9430674, PubMed:19539605). Plays a role in neuronal progenitor cell differentiation; induces neurite outgrowth via a AKT-dependent signaling cascade and calcium-independent manner (PubMed:23263657, PubMed:25450385). May recruit target proteins to the cell membrane in a calcium-dependent manner (PubMed:12522145). May function in membrane trafficking (PubMed:9430674). Involved in TNF-alpha-induced NF-kappa-B transcriptional repression by inducing endoprotease processing of the transcription factor NF-kappa-B p65/RELA subunit (PubMed:18212740). Also induces endoprotease processing of NF-kappa-B p50/NFKB1, p52/NFKB2, RELB and REL (PubMed:18212740).
NCBI Summary:	Calcium-dependent membrane-binding proteins may regulate molecular events at the interface of the cell membrane and cytoplasm. This gene encodes a calcium-dependent protein that also contains two N-terminal type II C2 domains and an integrin A domain-like sequence in the C-terminus. However, the encoded protein does not contain a predicted signal sequence or transmembrane domains. This protein has a broad tissue distribution and it may function in membrane trafficking. This gene and the gene for RNA binding motif protein 12 overlap at map location 20q11.21. Alternate splicing results in multiple transcript variants encoding different proteins. [provided by RefSeq, Aug 2008]
UniProt Code:	Q99829
NCBI GenInfo Identifier:	197333761
NCBI Gene ID:	8904
NCBI Accession:	NP_003906.2
UniProt Secondary Accession:	Q99829,Q6IBL3, Q9H243, Q9NTZ7, E1P5Q4,
UniProt Related Accession:	Q99829
Molecular Weight:	59,059 Da
NCBI Full Name:	copine-1 isoform b
NCBI Synonym Full Names:	copine 1
NCBI Official Symbol:	CPNE1
NCBI Official Synonym Symbols:	CPN1; COPN1
NCBI Protein Information:	copine-1
UniProt Protein Name:	Copine-1
UniProt Synonym Protein Names:	Chromobindin 17
Protein Family:	Carboxypeptidase
UniProt Gene Name:	CPNE1

Western blot analysis of extracts of various cell lines, using CPNE1 antibody (CAB15749) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.

Antibodies

CPNE1 Antibody (PACO08571)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)