Cell Biology Antibodies 5

Anti-COX15 Antibody (CAB14665)

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SKU:
CAB14665
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-COX15 Antibody
Antibody SKU:CAB14665
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 150-250 of human COX15 (NP_510870.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:U-251MG, A-431, Jurkat, Mouse heart, Mouse kidney, Mouse lung, Rat testis
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 150-250 of human COX15 (NP_510870.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:YSHR MWGR LVGL VYIL PAAY FWRK GWLS RGMK GRVL ALCG LVCF QGLL GWYM VKSG LEEK SDSH DIPR VSQY RLAA HLGS ALVL YCAS LWTS LSLL LPPH K
Gene ID:1355
Uniprot:Q7KZN9
Cellular Location:Mitochondrion membrane, Multi-pass membrane protein
Calculated MW:43kDa/46kDa
Observed MW:60kDa
Synonyms:COX15, CEMCOX2
Background:Cytochrome c oxidase (COX), the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes a protein which is not a structural subunit, but may be essential for the biogenesis of COX formation and may function in the hydroxylation of heme O, according to the yeast mutant studies. This protein is predicted to contain 5 transmembrane domains localized in the mitochondrial inner membrane. Alternative splicing of this gene generates two transcript variants diverging in the 3' region.
UniProt Protein Function:COX15: May be involved in the biosynthesis of heme A. Defects in COX15 are a cause of mitochondrial complex IV deficiency (MT-C4D); also known as cytochrome c oxidase deficiency. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations, ranging from isolated myopathy to severe multisystem disease affecting several tissues and organs. Features include hypertrophic cardiomyopathy, hepatomegaly and liver dysfunction, hypotonia, muscle weakness, excercise intolerance, developmental delay, delayed motor development and mental retardation. A subset of patients manifest Leigh syndrome. Defects in COX15 are a cause of Leigh syndrome (LS). An early-onset progressive neurodegenerative disorder characterized by the presence of focal, bilateral lesions in one or more areas of the central nervous system including the brainstem, thalamus, basal ganglia, cerebellum and spinal cord. Clinical features depend on which areas of the central nervous system are involved and include subacute onset of psychomotor retardation, hypotonia, ataxia, weakness, vision loss, eye movement abnormalities, seizures, and dysphagia. Belongs to the COX15/CtaA family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; Membrane protein, integral; Energy Metabolism - oxidative phosphorylation; Membrane protein, multi-pass; Mitochondrial

Chromosomal Location of Human Ortholog: 10q24

Cellular Component: mitochondrion; mitochondrial inner membrane; integral to membrane; mitochondrial respiratory chain

Molecular Function:cytochrome-c oxidase activity; oxidoreductase activity, acting on the CH-CH group of donors

Biological Process: respiratory chain complex IV assembly; porphyrin metabolic process; cellular respiration; mitochondrial electron transport, cytochrome c to oxygen; respiratory gaseous exchange; heme a biosynthetic process; heme biosynthetic process

Disease: Leigh Syndrome; Cardioencephalomyopathy, Fatal Infantile, Due To Cytochrome C Oxidase Deficiency 2

NCBI Summary:Cytochrome c oxidase (COX), the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes a protein which is not a structural subunit, but may be essential for the biogenesis of COX formation and may function in the hydroxylation of heme O, according to the yeast mutant studies. This protein is predicted to contain 5 transmembrane domains localized in the mitochondrial inner membrane. Alternative splicing of this gene generates two transcript variants diverging in the 3' region. [provided by RefSeq, Jul 2008]
UniProt Code:Q7KZN9
NCBI GenInfo Identifier:17921987
NCBI Gene ID:1355
NCBI Accession:NP_004367.2
UniProt Secondary Accession:Q7KZN9,O60556, O75878, Q5TD00, Q5TD01, Q7Z3Q3, Q9NTN0 A8K6I9,
UniProt Related Accession:Q7KZN9
Molecular Weight:43,835 Da
NCBI Full Name:cytochrome c oxidase assembly protein COX15 homolog isoform 2
NCBI Synonym Full Names:cytochrome c oxidase assembly homolog 15 (yeast)
NCBI Official Symbol:COX15  
NCBI Official Synonym Symbols:CEMCOX2  
NCBI Protein Information:cytochrome c oxidase assembly protein COX15 homolog; cytochrome c oxidase subunit 15; COX15 homolog, cytochrome c oxidase assembly protein
UniProt Protein Name:Cytochrome c oxidase assembly protein COX15 homolog
Protein Family:Cytochrome c oxidase assembly protein
UniProt Gene Name:COX15  
UniProt Entry Name:COX15_HUMAN
Anti-COX15 Antibody (CAB14665)Western blot analysis of extracts of various cell lines, using COX15 antibody (CAB14665) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Anti-COX15 Antibody (CAB14665)Immunohistochemistry of paraffin-embedded rat spleen using COX15 antibody (CAB14665) at dilution of 1:100 (40x lens).
Anti-COX15 Antibody (CAB14665)Immunohistochemistry of paraffin-embedded human thyroid cancer using COX15 antibody (CAB14665) at dilution of 1:100 (40x lens).
Anti-COX15 Antibody (CAB14665)Immunohistochemistry of paraffin-embedded mouse heart using COX15 antibody (CAB14665) at dilution of 1:100 (40x lens).
Anti-COX15 Antibody (CAB14665)Immunofluorescence analysis of L929 cells using COX15 Polyclonal Antibody (CAB14665) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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