Cell Biology Antibodies 6
Anti-COPG2 Antibody (CAB15817)
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- SKU:
- CAB15817
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-COPG2 Antibody |
| Antibody SKU: | CAB15817 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 542-871 of human COPG2 (NP_036265.3). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse heart, Mouse kidney, Rat heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 542-871 of human COPG2 (NP_036265.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LNAT YIFN GLTV SVPG MEKA LHQY TLEP SEKP FDMK SIPL AMAP VFEQ KAEI TLVA TKPE KLAP SRQD IFQE QLAA IPEF LNIG PLFK SSEP VQLT EAET EYFV RCIK HMFT NHIV FQFD CTNT LNDQ LLEK VTVQ MEPS DSYE VLSC IPAP SLPY NQPG ICYT LVRL PDDD PTAV AGSF SCTM KFTV RDCD PNTG VPDE DGYD DEYV LEDL EVTV SDHI QKVL KPNF AAAW EEVG DTFE KEET FALS STKT LEEA VNNI ITFL GMQP CERS DKVP ENKN SHSL YLAG IFRG GYDL LVRS RLAL ADGV TMQV TVRS KERT PVDV ILAS VG |
| Gene ID: | 26958 |
| Uniprot: | Q9UBF2 |
| Cellular Location: | COPI-coated vesicle membrane, Cytoplasm, Cytoplasmic side, Cytoplasmic vesicle, Golgi apparatus membrane, Peripheral membrane protein, cytosol |
| Calculated MW: | 81kDa/97kDa |
| Observed MW: | 105kDa |
| Synonyms: | COPG2, 2-COP, gamma-2-COP |
| Background: |
| UniProt Protein Function: | COPG2: The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors. Belongs to the COPG family. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Vesicle Chromosomal Location of Human Ortholog: 7q32 Cellular Component: COPI vesicle coat; cytosol Molecular Function:structural molecule activity Biological Process: intra-Golgi vesicle-mediated transport; intracellular protein transport; retrograde vesicle-mediated transport, Golgi to ER |
| UniProt Code: | Q9UBF2 |
| NCBI GenInfo Identifier: | 13124090 |
| NCBI Gene ID: | 26958 |
| NCBI Accession: | Q9UBF2.1 |
| UniProt Related Accession: | Q9UBF2 |
| Molecular Weight: | Calculated: 81kDa; 97kDaObserved: 105kDa |
| NCBI Full Name: | Coatomer subunit gamma-2 |
| NCBI Synonym Full Names: | coatomer protein complex subunit gamma 2 |
| NCBI Official Symbol: | COPG2 |
| NCBI Official Synonym Symbols: | 2-COP; gamma-2-COP |
| NCBI Protein Information: | coatomer subunit gamma-2 |
| UniProt Protein Name: | Coatomer subunit gamma-2 |
| UniProt Synonym Protein Names: | Gamma-2-coat protein; Gamma-2-COP |
| Protein Family: | Coatomer |
| UniProt Gene Name: | COPG2 |
| UniProt Entry Name: | COPG2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using COPG2 antibody (CAB15817) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded mouse brain using COPG2 antibody (CAB15817) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U-2 OS cells using COPG2 Polyclonal Antibody (CAB15817) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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