Antibody Name:	C5/C5a Rabbit Polyclonal Antibody
Antibody SKU:	CAB8104
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1567-1676 of human C5/C5a (NP_001726.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-937, Jurkat, Mouse liver, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1567-1676 of human C5/C5a (NP_001726.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SITV ENVF VKYK ATLL DIYK TGEA VAEK DSEI TFIK KVTC TNAE LVKG RQYL IMGK EALQ IKYN FSFR YIYP LDSL TWIE YWPR DTTC SSCQ AFLA NLDE FAED IFLN GC
Gene ID:	727
Uniprot:	P01031
Cellular Location:	Secreted
Calculated MW:	188kDa
Observed MW:	110kDa/180kDa

Synonyms:

C5, C5D, C5a, C5b, CPAMD4, ECLZB

Background:

This gene encodes a component of the complement system, a part of the innate immune system that plays an important role in inflammation, host homeostasis, and host defense against pathogens. The encoded preproprotein is proteolytically processed to generate multiple protein products, including the C5 alpha chain, C5 beta chain, C5a anaphylatoxin and C5b. The C5 protein is comprised of the C5 alpha and beta chains, which are linked by a disulfide bridge. Cleavage of the alpha chain by a convertase enzyme results in the formation of the C5a anaphylatoxin, which possesses potent spasmogenic and chemotactic activity, and the C5b macromolecular cleavage product, a subunit of the membrane attack complex (MAC). Mutations in this gene cause complement component 5 deficiency, a disease characterized by recurrent bacterial infections. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	C5: Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. Defects in C5 are the cause of complement component 5 deficiency (C5D). A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis. An association study of C5 haplotypes and genotypes in individuals with chronic hepatitis C virus infection shows that individuals homozygous for the C5_1 haplotype have a significantly higher stage of liver fibrosis than individuals carrying at least 1 other allele (PubMed:15995705).
UniProt Protein Details:	Protein type:Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 9q33-q34 Cellular Component: membrane attack complex; extracellular space; extracellular region Molecular Function:protein binding; chemokine activity; endopeptidase inhibitor activity; C5a anaphylatoxin chemotactic receptor binding; receptor binding Biological Process: in utero embryonic development; positive regulation of chemotaxis; activation of MAPK activity; cytolysis; complement activation, alternative pathway; chemotaxis; glucose homeostasis; leukocyte migration during inflammatory response; complement activation; cellular calcium ion homeostasis; G-protein coupled receptor protein signaling pathway; positive regulation of angiogenesis; cell surface receptor linked signal transduction; regulation of complement activation; response to stress; innate immune response; negative regulation of dopamine secretion; inflammatory response; complement activation, classical pathway Disease: Complement Component 5 Deficiency; Eculizumab, Poor Response To
NCBI Summary:	The protein encoded by this gene is the fifth component of complement, which plays an important role in inflammatory and cell killing processes. This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase. The C5b macromolecular cleavage product can form a complex with the C6 complement component, and this complex is the basis for formation of the membrane attack complex, which includes additional complement components. Mutations in this gene cause complement component 5 deficiency, a disease where patients show a propensity for severe recurrent infections. Defects in this gene have also been linked to a susceptibility to liver fibrosis and to rheumatoid arthritis. [provided by RefSeq, Jul 2008]
UniProt Code:	P01031
NCBI GenInfo Identifier:	166900096
NCBI Gene ID:	727
NCBI Accession:	P01031.4
UniProt Secondary Accession:	P01031,Q14CJ0, Q27I61,
UniProt Related Accession:	P01031
Molecular Weight:	188,305 Da
NCBI Full Name:	Complement C5
NCBI Synonym Full Names:	complement component 5
NCBI Official Symbol:	C5
NCBI Official Synonym Symbols:	C5D; C5a; C5b; ECLZB; CPAMD4
NCBI Protein Information:	complement C5; prepro-C5; C5a anaphylatoxin; anaphylatoxin C5a analog; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4
UniProt Protein Name:	Complement C5
UniProt Synonym Protein Names:	C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4Cleaved into the following 4 chains:Complement C5 beta chain; Complement C5 alpha chain; C5a anaphylatoxin; Complement C5 alpha' chain
Protein Family:	Complement C5
UniProt Gene Name:	C5
UniProt Entry Name:	CO5_HUMAN

	Western blot analysis of extracts of various cell lines, using C5/C5a pAb (CAB8104) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s.
	Western blot analysis of extracts of various cell lines, using C5/C5a pAb (CAB8104) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human stomach using C5/C5a antibody (CAB8104) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using C5/C5a antibody (CAB8104) at dilution of 1:100. Blue: DAPI for nuclear staining.