Cell Death Antibodies 2

Anti-COMP Antibody (CAB5812)

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SKU:
CAB5812
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death

Description

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Antibody Name:Anti-COMP Antibody
Antibody SKU:CAB5812
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 20-160 of human COMP (NP_000086.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:10 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:SW620, U-251MG, HepG2, MCF7, Mouse skeletal muscle
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 20-160 of human COMP (NP_000086.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:GQGQ SPLG SDLG PQML RELQ ETNA ALQD VREL LRQQ VREI TFLK NTVM ECDA CGMQ QSVR TGLP SVRP LLHC APGF CFPG VACI QTES GARC GPCP AGFT GNGS HCTD VNEC NAHP CFPR VRCI NTSP GFRC EACP PGYS G
Gene ID:1311
Uniprot:P49747
Cellular Location:Secreted, extracellular matrix, extracellular space
Calculated MW:77kDa/82kDa
Observed MW:110kDa
Synonyms:COMP, EDM1, EPD1, MED, PSACH, THBS5, TSP5
Background:The protein encoded by this gene is a noncollagenous extracellular matrix (ECM) protein. It consists of five identical glycoprotein subunits, each with EGF-like and calcium-binding (thrombospondin-like) domains. Oligomerization results from formation of a five-stranded coiled coil and disulfides. Binding to other ECM proteins such as collagen appears to depend on divalent cations. Contraction or expansion of a 5 aa aspartate repeat and other mutations can cause pseudochondroplasia (PSACH) and multiple epiphyseal dysplasia (MED).
UniProt Protein Function:COMP: May play a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors. Could play a role in the pathogenesis of osteoarthritis. Potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Suppresses apoptosis by blocking the activation of caspase-3 and by inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5 and XIAP). Essential for maintaining a vascular smooth muscle cells (VSMCs) contractile/differentiated phenotype under physiological and pathological stimuli. Maintains this phenotype of VSMCs by interacting with ITGA7. Defects in COMP are the cause of multiple epiphyseal dysplasia type 1 (EDM1). EDM is a generalized skeletal dysplasia associated with significant morbidity. Joint pain, joint deformity, waddling gait, and short stature are the main clinical signs and symptoms. EDM is broadly categorized into the more severe Fairbank and the milder Ribbing types. Defects in COMP are the cause of pseudoachondroplasia (PSACH). PSAC is a dominantly inherited chondrodysplasia characterized by short stature and early-onset osteoarthrosis. PSACH is more severe than EDM1 and is recognized in early childhood. Belongs to the thrombospondin family.
UniProt Protein Details:

Protein type:Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; extracellular region

Molecular Function:heparin binding; heparan sulfate proteoglycan binding; collagen binding; protein binding; protease binding; extracellular matrix structural constituent; calcium ion binding

Biological Process: limb development; organ morphogenesis; extracellular matrix organization and biogenesis; apoptosis; cell adhesion; skeletal development; negative regulation of apoptosis

Disease: Pseudoachondroplasia; Epiphyseal Dysplasia, Multiple, 1

NCBI Summary:The protein encoded by this gene is a noncollagenous extracellular matrix (ECM) protein. It consists of five identical glycoprotein subunits, each with EGF-like and calcium-binding (thrombospondin-like) domains. Oligomerization results from formation of a five-stranded coiled coil and disulfides. Binding to other ECM proteins such as collagen appears to depend on divalent cations. Mutations can cause the osteochondrodysplasias pseudochondroplasia (PSACH) and multiple epiphyseal dysplasia (MED). [provided by RefSeq, Jul 2008]
UniProt Code:P49747
NCBI GenInfo Identifier:209572601
NCBI Gene ID:1311
NCBI Accession:P49747.2
UniProt Secondary Accession:P49747,O14592, Q16388, Q16389, Q2NL86, Q8N4T2, B4DKJ3
UniProt Related Accession:P49747
Molecular Weight:77,214 Da
NCBI Full Name:Cartilage oligomeric matrix protein
NCBI Synonym Full Names:cartilage oligomeric matrix protein
NCBI Official Symbol:COMP  
NCBI Official Synonym Symbols:MED; EDM1; EPD1; PSACH; THBS5  
NCBI Protein Information:cartilage oligomeric matrix protein; TSP5; thrombospondin-5; pseudoachondroplasia (epiphyseal dysplasia 1, multiple); cartilage oligomeric matrix protein(pseudoachondroplasia, epiphyseal dysplasia 1, multiple); cartilage oligomeric matrix protein (pseudoachondroplasia, epiphyseal dysplasia 1, multiple)
UniProt Protein Name:Cartilage oligomeric matrix protein
UniProt Synonym Protein Names:Thrombospondin-5; TSP5
Protein Family:Complexin
UniProt Gene Name:COMP  
UniProt Entry Name:COMP_HUMAN
Anti-COMP Antibody (CAB5812)Western blot analysis of extracts of various cell lines, using COMP antibody (CAB5812) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s.
Anti-COMP Antibody (CAB5812)Immunofluorescence analysis of U2OS cells using COMP antibody (CAB5812). Blue: DAPI for nuclear staining.
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