Name: Anti-COL5A1 Antibody (CAB1515)
Brand: Cell Biology Antibodies 5
SKU: CAB1515
Availability: OutOfStock

Antibody Name:	Anti-COL5A1 Antibody
Antibody SKU:	CAB1515
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Mouse
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 20-120 of human COL5A1 (NP_000084.3).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Mouse
Positive Samples:	Mouse lung

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 20-120 of human COL5A1 (NP_000084.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LPPL LLLL LWAP PPSR AAQP ADLL KVLD FHNL PDGI TKTT GFCA TRRS SKGP DVAY RVTK DAQL SAPT KQLY PASA FPED FSIL TTVK AKKG SQAF LVSI Y
Gene ID:	1289
Uniprot:	P20908
Cellular Location:	Secreted, extracellular matrix, extracellular space
Calculated MW:	183kDa
Observed MW:	180kDa

Synonyms:

COL5A1, EDSC

Background:

This gene encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. The encoded procollagen protein occurs commonly as the heterotrimer pro-alpha1(V)-pro-alpha1(V)-pro-alpha2(V). Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II. Alternative splicing of this gene results in multiple transcript variants.

UniProt Protein Function:	COL5A1: Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin. Defects in COL5A1 are a cause of Ehlers-Danlos syndrome type 1 (EDS1); also known as Ehlers-Danlos syndrome gravis or severe classic type Ehlers-Danlos syndrome. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome. Defects in COL5A1 are a cause of Ehlers-Danlos syndrome type 2 (EDS2); also known as Ehlers-Danlos syndrome mitis or mild classic type Ehlers Danlos syndrome. Belongs to the fibrillar collagen family.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Secreted; Cell adhesion; Secreted, signal peptide; Extracellular matrix Chromosomal Location of Human Ortholog: 9q34.2-q34.3 Cellular Component: extracellular matrix; collagen type V; endoplasmic reticulum lumen; extracellular region; basement membrane Molecular Function:heparin binding; integrin binding; proteoglycan binding; platelet-derived growth factor binding; extracellular matrix structural constituent; metal ion binding Biological Process: skin development; heart morphogenesis; blood vessel development; axon guidance; extracellular matrix organization and biogenesis; cell migration; collagen fibril organization; extracellular matrix disassembly; collagen catabolic process; integrin biosynthetic process; regulation of cellular component organization and biogenesis; fibril organization and biogenesis; cell adhesion; collagen biosynthetic process; eye morphogenesis; wound healing, spreading of epidermal cells Disease: Ehlers-danlos Syndrome, Type I
NCBI Summary:	This gene encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. The encoded procollagen protein occurs commonly as the heterotrimer pro-alpha1(V)-pro-alpha1(V)-pro-alpha2(V). Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013]
UniProt Code:	P20908
NCBI GenInfo Identifier:	89276751
NCBI Gene ID:	1289
NCBI Accession:	NP_000084.3
UniProt Secondary Accession:	P20908,Q15094, Q5SUX4,
UniProt Related Accession:	P20908
Molecular Weight:	183,560 Da
NCBI Full Name:	collagen alpha-1(V) chain isoform 1 preproprotein
NCBI Synonym Full Names:	collagen, type V, alpha 1
NCBI Official Symbol:	COL5A1
NCBI Protein Information:	collagen alpha-1(V) chain; collagen alpha-1(V) chain
UniProt Protein Name:	Collagen alpha-1(V) chain
Protein Family:	Collagen
UniProt Gene Name:	COL5A1
UniProt Entry Name:	CO5A1_HUMAN

Western blot analysis of extracts of mouse lung, using COL5A1 antibody (CAB1515) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.