Cell Biology Antibodies 6

Anti-COG4 Antibody (CAB16111)

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SKU:
CAB16111
Product Type:
Antibody
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-COG4 Antibody
Antibody SKU:CAB16111
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 310-410 of human COG4 (NP_056201.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:Mouse, Rat
Positive Samples:Mouse brain, Mouse kidney, Mouse liver, Mouse lung, Rat pancreas
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 310-410 of human COG4 (NP_056201.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LQVE CDRQ VEKV VDKF IKQR DYHQ QFRH VQNN LMRN STTE KIEP RELD PILT EVTL MNAR SELY LRFL KKRI SSDF EVGD SMAS EEVK QEHQ KCLD KLLN N
Gene ID:25839
Uniprot:Q9H9E3
Cellular Location:Cytoplasmic side, Golgi apparatus membrane, Peripheral membrane protein
Calculated MW:38kDa/81kDa/89kDa
Observed MW:100kDa
Synonyms:COG4, CDG2J, COD1
Background:The protein encoded by this gene is a component of an oligomeric protein complex involved in the structure and function of the Golgi apparatus. Defects in this gene may be a cause of congenital disorder of glycosylation type IIj. Two transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:COG4: Required for normal Golgi function. Plays a role in SNARE-pin assembly and Golgi-to-ER retrograde transport via its interaction with SCFD1. Defects in COG4 are the cause of congenital disorder of glycosylation type 2J (CDG2J). It is a multisystem disorder caused by a defect in glycoprotein biosynthesis and characterized by under-glycosylated serum glycoproteins. Congenital disorders of glycosylation result in a wide variety of clinical features, such as defects in the nervous system development, psychomotor retardation, dysmorphic features, hypotonia, coagulation disorders, and immunodeficiency. The broad spectrum of features reflects the critical role of N-glycoproteins during embryonic development, differentiation, and maintenance of cell functions. Belongs to the COG4 family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Vesicle; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: Golgi membrane; Golgi transport complex; trans-Golgi network membrane

Molecular Function:protein binding

Biological Process: ER to Golgi vesicle-mediated transport; Golgi organization and biogenesis; Golgi vesicle prefusion complex stabilization; retrograde transport, vesicle recycling within Golgi; retrograde vesicle-mediated transport, Golgi to ER

Disease: Congenital Disorder Of Glycosylation, Type Iij

NCBI Summary:The protein encoded by this gene is a component of an oligomeric protein complex involved in the structure and function of the Golgi apparatus. Defects in this gene may be a cause of congenital disorder of glycosylation type IIj. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2010]
UniProt Code:Q9H9E3
NCBI GenInfo Identifier:311033464
NCBI Gene ID:25839
NCBI Accession:Q9H9E3.3
UniProt Secondary Accession:Q9H9E3,Q96D40, Q9BRF0, Q9BVZ2, Q9H5Y4, Q9Y3W3, B4DMN8 C9JS23,
UniProt Related Accession:Q9H9E3
Molecular Weight:81,098 Da
NCBI Full Name:Conserved oligomeric Golgi complex subunit 4
NCBI Synonym Full Names:component of oligomeric golgi complex 4
NCBI Official Symbol:COG4  
NCBI Official Synonym Symbols:COD1; CDG2J  
NCBI Protein Information:conserved oligomeric Golgi complex subunit 4
UniProt Protein Name:Conserved oligomeric Golgi complex subunit 4
UniProt Synonym Protein Names:Component of oligomeric Golgi complex 4
Protein Family:Conserved oligomeric Golgi complex
UniProt Gene Name:COG4  
UniProt Entry Name:COG4_HUMAN
Anti-COG4 Antibody (CAB16111)Western blot analysis of extracts of various cell lines, using COG4 antibody (CAB16111) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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