Epigenetics & Nuclear Signaling Antibodies 1

Anti-COASY Antibody (CAB12179)

(No reviews yet) Write a Review
SKU:
CAB12179
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-COASY Antibody
Antibody SKU:CAB12179
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 345-564 of human COASY (NP_079509.5).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:LO2, 293T, A-549, SKOV3, Mouse heart, Mouse liver, Rat kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 345-564 of human COASY (NP_079509.5).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LRPP YERP ELPT CLYV IGLT GISG SGKS SIAQ RLKG LGAF VIDS DHLG HRAY APGG PAYQ PVVE AFGT DILH KDGI INRK VLGS RVFG NKKQ LKIL TDIM WPII AKLA REEM DRAV AEGK RVCV IDAA VLLE AGWQ NLVH EVWT AVIP ETEA VRRI VERD GLSE AAAQ SRLQ SQMS GQQL VEQS HVVL STLW EPHI TQRQ VEKA WALL QKRI PKTH QALD
Gene ID:80347
Uniprot:Q13057
Cellular Location:Cytoplasm, Mitochondrion matrix
Calculated MW:62kDa/65kDa
Observed MW:62kDa
Synonyms:COASY, DPCK, NBIA6, NBP, PPAT, UKR1, pOV-2
Background:Coenzyme A (CoA) functions as a carrier of acetyl and acyl groups in cells and thus plays an important role in numerous synthetic and degradative metabolic pathways in all organisms. In eukaryotes, CoA and its derivatives are also involved in membrane trafficking and signal transduction. This gene encodes the bifunctional protein coenzyme A synthase (CoAsy) which carries out the last two steps in the biosynthesis of CoA from pantothenic acid (vitamin B5). The phosphopantetheine adenylyltransferase domain of this bifunctional protein catalyzes the conversion of 4'-phosphopantetheine into dephospho-coenzyme A (dpCoA) while its dephospho-CoA kinase domain completes the final step by phosphorylating dpCoA to form CoA. Mutations in this gene are associated with neurodegeneration with brain iron accumulation (NBIA). Alternative splicing results in multiple isoforms.
UniProt Protein Function:PPAT: bifunctional cytoplasmic enzyme that catalyzes the fourth and fifth sequential steps of CoA biosynthetic pathway. The fourth reaction is catalyzed by the phosphopantetheine adenylyltransferase, coded by the coaD domain; the fifth reaction is catalyzed by the dephospho-CoA kinase, coded by the coaE domain. May act as a point of CoA biosynthesis regulation.
UniProt Protein Details:

Protein type:Kinase, other; EC 2.7.7.3; Transferase; EC 2.7.1.24; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis

Chromosomal Location of Human Ortholog: 17q12-q21

Cellular Component: nucleoplasm; mitochondrial outer membrane; mitochondrial matrix; cytoplasm

Molecular Function:protein binding; dephospho-CoA kinase activity; pantetheine-phosphate adenylyltransferase activity; ATP binding

Biological Process: coenzyme A biosynthetic process; coenzyme biosynthetic process; vitamin metabolic process; pantothenate metabolic process; phosphorylation; water-soluble vitamin metabolic process

Disease: Neurodegeneration With Brain Iron Accumulation 6

NCBI Summary:Coenzyme A (CoA) functions as a carrier of acetyl and acyl groups in cells and thus plays an important role in numerous synthetic and degradative metabolic pathways in all organisms. In eukaryotes, CoA and its derivatives are also involved in membrane trafficking and signal transduction. This gene encodes the bifunctional protein coenzyme A synthase (CoAsy) which carries out the last two steps in the biosynthesis of CoA from pantothenic acid (vitamin B5). The phosphopantetheine adenylyltransferase domain of this bifunctional protein catalyzes the conversion of 4'-phosphopantetheine into dephospho-coenzyme A (dpCoA) while its dephospho-CoA kinase domain completes the final step by phosphorylating dpCoA to form CoA. Mutations in this gene are associated with neurodegeneration with brain iron accumulation (NBIA). Alternative splicing results in multiple isoforms. [provided by RefSeq, Apr 2014]
UniProt Code:Q13057
NCBI GenInfo Identifier:32363505
NCBI Gene ID:80347
NCBI Accession:Q13057.4
UniProt Related Accession:Q13057
Molecular Weight:
NCBI Full Name:Bifunctional coenzyme A synthase
NCBI Synonym Full Names:Coenzyme A synthase
NCBI Official Symbol:COASY  
NCBI Official Synonym Symbols:NBP; DPCK; PPAT; UKR1; NBIA6; PCH12; pOV-2  
NCBI Protein Information:bifunctional coenzyme A synthase
UniProt Protein Name:Bifunctional coenzyme A synthase
UniProt Synonym Protein Names:NBP; POV-2Including the following 2 domains:Phosphopantetheine adenylyltransferase (EC:2.7.7.3)Alternative name(s):Dephospho-CoA pyrophosphorylase; Pantetheine-phosphate adenylyltransferase; PPAT
Protein Family:Bifunctional coenzyme A synthase
UniProt Gene Name:COASY  
UniProt Entry Name:COASY_HUMAN
Anti-COASY Antibody (CAB12179)Western blot analysis of extracts of various cell lines, using COASY antibody (CAB12179) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
View AllClose

0 Reviews

View AllClose

Related Products