Antibody Name:	Anti-CNTF Antibody
Antibody SKU:	CAB1915
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human CNTF (NP_000605.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse skeletal muscle

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human CNTF (NP_000605.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAFT EHSP LTPH RRDL CSRS IWLA RKIR SDLT ALTE SYVK HQGL NKNI NLDS ADGM PVAS TDQW SELT EAER LQEN LQAY RTFH VLLA RLLE DQQV HFTP TEGD FHQA IHTL LLQV AAFA YQIE ELMI LLEY KIPR NEAD GMPI NVGD GGLF EKKL WGLK VLQE LSQW TVRS IHDL RFIS SHQT GIPA RGSH YIAN NKKM
Gene ID:	1270
Uniprot:	P26441
Cellular Location:	Cytoplasm
Calculated MW:	22kDa
Observed MW:	26kDa

Synonyms:

CNTF, HCNTF

Background:

The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. A read-through transcript variant composed of the upstream ZFP91 gene and CNTF sequence has been identified, but it is thought to be non-coding. Read-through transcription of ZFP91 and CNTF has also been observed in mouse.

UniProt Protein Function:	CNTF: CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy. Belongs to the CNTF family.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 11q12.2 Cellular Component: cytoplasm; extracellular region; extracellular space Molecular Function:ciliary neurotrophic factor receptor binding; growth factor activity; interleukin-6 receptor binding; protein binding Biological Process: growth; muscle morphogenesis; negative regulation of neuron apoptosis; negative regulation of photoreceptor cell differentiation; neuron development; positive regulation of cell proliferation; positive regulation of tyrosine phosphorylation of Stat3 protein; regulation of retinal cell programmed cell death; signal transduction
NCBI Summary:	The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. A read-through transcript variant composed of the upstream ZFP91 gene and CNTF sequence has been identified, but it is thought to be non-coding. Read-through transcription of ZFP91 and CNTF has also been observed in mouse. [provided by RefSeq, Oct 2010]
UniProt Code:	P26441
NCBI GenInfo Identifier:	116585
NCBI Gene ID:	1270
NCBI Accession:	P26441.1
UniProt Secondary Accession:	P26441,B2RAB2,
UniProt Related Accession:	P26441
Molecular Weight:
NCBI Full Name:	Ciliary neurotrophic factor
NCBI Synonym Full Names:	ciliary neurotrophic factor
NCBI Official Symbol:	CNTF
NCBI Official Synonym Symbols:	HCNTF
NCBI Protein Information:	ciliary neurotrophic factor
UniProt Protein Name:	Ciliary neurotrophic factor
Protein Family:	Ciliary neurotrophic factor
UniProt Gene Name:	CNTF
UniProt Entry Name:	CNTF_HUMAN

	Western blot analysis of extracts of mouse muscle, using CNTF antibody (CAB1915) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunofluorescence analysis of C6 cells using CNTF Rabbit pAb (CAB1915) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of mouse spinal cord using CNTF Rabbit pAb (CAB1915) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.