Epigenetics & Nuclear Signaling Antibodies 1

Anti-CNOT2 Antibody (CAB12832)

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SKU:
CAB12832
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-CNOT2 Antibody
Antibody SKU:CAB12832
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 341-540 of human CNOT2 (NP_055330.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:HT-29, Mouse brain, Mouse heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 341-540 of human CNOT2 (NP_055330.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:GRVT NIPQ GMVT DQFG MIGL LTFI RAAE TDPG MVHL ALGS DLTT LGLN LNSP ENLY PKFA SPWA SSPC RPQD IDFH VPSE YLTN IHIR DKLA AIKL GRYG EDLL FYLY YMNG GDVL QLLA AVEL FNRD WRYH KEER VWIT RAPG MEPT MKTN TYER GTYY FFDC LNWR KVAK EFHL EYDK LEER PHLP STFN YNPA QQAF
Gene ID:4848
Uniprot:Q9NZN8
Cellular Location:Cytoplasm, Nucleus
Calculated MW:22kDa/40kDa/51kDa/54kDa/59kDa
Observed MW:75kDa
Synonyms:CNOT2, CDC36, HSPC131, NOT2, NOT2H
Background:This gene encodes a subunit of the multi-component CCR4-NOT complex. The CCR4-NOT complex regulates mRNA synthesis and degradation and is also thought to be involved in mRNA splicing, transport and localization. The encoded protein interacts with histone deacetylases and functions as a repressor of polymerase II transcription. Alternatively spliced transcript variants have been observed for this gene.
UniProt Protein Function:NOT2: The CCR4-NOT complex functions as general transcription regulation complex. Subunit of the CCR4-NOT core complex that contains CHAF1A, CHAF1B, CNOT1, CNOT2, CNOT3, CNOT4, CNOT6 and CNOT8. Ubiquitous. Highly expressed in brain, heart, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood leukocytes. Belongs to the CNOT2/3/5 family. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 12q15

Cellular Component: CCR4-NOT complex; cytoplasm; cytosol; membrane; nucleus; plasma membrane

Molecular Function:poly(A)-specific ribonuclease activity; protein binding

Biological Process: DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; mRNA catabolic process, deadenylation-dependent decay; negative regulation of estrogen receptor signaling pathway; negative regulation of transcription from RNA polymerase II promoter; negative regulation of translation; poly(A) tail shortening

NCBI Summary:This gene encodes a subunit of the multi-component CCR4-NOT complex. The CCR4-NOT complex regulates mRNA synthesis and degradation and is also thought to be involved in mRNA splicing, transport and localization. The encoded protein interacts with histone deacetylases and functions as a repressor of polymerase II transcription. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Dec 2010]
UniProt Code:Q9NZN8
NCBI GenInfo Identifier:46396017
NCBI Gene ID:4848
NCBI Accession:Q9NZN8.1
UniProt Secondary Accession:Q9NZN8,Q9H3E0, Q9NSX5, Q9NWR6, Q9P028,
UniProt Related Accession:Q9NZN8
Molecular Weight:60kDa
NCBI Full Name:CCR4-NOT transcription complex subunit 2
NCBI Synonym Full Names:CCR4-NOT transcription complex subunit 2
NCBI Official Symbol:CNOT2  
NCBI Official Synonym Symbols:NOT2; CDC36; NOT2H; HSPC131  
NCBI Protein Information:CCR4-NOT transcription complex subunit 2
UniProt Protein Name:CCR4-NOT transcription complex subunit 2
UniProt Synonym Protein Names:CCR4-associated factor 2
Protein Family:CCR4-NOT transcription complex
UniProt Gene Name:CNOT2  
Anti-CNOT2 Antibody (CAB12832)Western blot analysis of extracts of various cell lines, using CNOT2 antibody (CAB12832) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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