Epigenetics & Nuclear Signaling Antibodies 1
Anti-CNOT10 Antibody (CAB12164)
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- SKU:
- CAB12164
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-CNOT10 Antibody |
| Antibody SKU: | CAB12164 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 465-744 of human CNOT10 (NP_056257.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | A-431, 293T, LO2, NCl-H460, Mouse testis, Mouse thymus |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 465-744 of human CNOT10 (NP_056257.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LRNA LLLL PEEQ QDPK QENG AKNS NQLG GNTE SSES SETC SSKS HDGD KFIP APPS SPLR KQEL ENLK CSIL ACSA YVAL ALGD NLMA LNHA DKLL QQPK LSGS LKFL GHLY AAEA LISL DRIS DAIT HLNP ENVT DVSL GISS NEQD QGSD KGEN EAME SSGK RAPQ CYPS SVNS ARTV MLFN LGSA YCLR SEYD KARK CLHQ AASM IHPK EVPP EAIL LAVY LELQ NGNT QLAL QIIK RNQL LPAV KTHS EVRK KPVF QPVH PIQP IQMP AFTT VQRK |
| Gene ID: | 25904 |
| Uniprot: | Q9H9A5 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 49kDa/76kDa/79kDa/82kDa/89kDa |
| Observed MW: | 90kDa |
| Synonyms: | CNOT10 |
| Background: |
| UniProt Protein Function: | CNOT10: Belongs to the CNOT10 family. 5 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 3p22.3 Cellular Component: CCR4-NOT complex; cytosol; membrane Molecular Function:protein binding Biological Process: DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; poly(A) tail shortening |
| UniProt Code: | Q9H9A5 |
| NCBI GenInfo Identifier: | 74733982 |
| NCBI Gene ID: | 25904 |
| NCBI Accession: | Q9H9A5.1 |
| UniProt Secondary Accession: | Q9H9A5,Q9BU30, Q9H5J7, Q9H8X1, Q9H9W0, Q9HAH3, Q9UFJ2 B7Z7L1, F8WAF2, |
| UniProt Related Accession: | Q9H9A5 |
| Molecular Weight: | 82kDa |
| NCBI Full Name: | CCR4-NOT transcription complex subunit 10 |
| NCBI Synonym Full Names: | CCR4-NOT transcription complex subunit 10 |
| NCBI Official Symbol: | CNOT10 |
| NCBI Protein Information: | CCR4-NOT transcription complex subunit 10 |
| UniProt Protein Name: | CCR4-NOT transcription complex subunit 10 |
| Protein Family: | CCR4-NOT transcription complex |
| UniProt Gene Name: | CNOT10 |
 | Western blot analysis of extracts of various cell lines, using CNOT10 antibody (CAB12164) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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